Distribution and Activity of Sulfur-Metabolizing Bacteria along the Temperature Gradient in Phototrophic Mats of the Chilean Hot Spring Porcelana

• Published in: Microorganisms (Basel) 2023-07, Vol.11 (7), p.1803
• Main Authors: Konrad, Ricardo, Vergara-Barros, Pablo, Alcorta, Jaime, Alcamán-Arias, María E, Levicán, Gloria, Ridley, Christina, Díez, Beatriz
• Format: Article
• Language: English
• Subjects: Abundance; aprA; Bacteria; Bacteria, Photosynthetic; Burkholderiales; Calibration; Carbon cycle; Chemical elements; Chemical speciation; Chloroflexota; Climate change; Climatic changes; Cloning; Control; E coli; Environmental aspects; Enzymes; Genes; Global climate; Health aspects; High temperature; Hot springs; Identification and classification; Metabolism; metagenomics; Microbial mats; Microorganisms; Oxidation; phototrophic hot spring mat; Proteins; Reductases; soxB; Spring (season); Sulfate reduction; Sulfates; Sulfur; Sulfur cycle; sulfur oxidation/reduction; Taxonomy; Temperature; Temperature gradients; Thiosulfate; Thiosulfates
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms11071803

In terrestrial hot springs, some members of the microbial mat community utilize sulfur chemical species for reduction and oxidization metabolism. In this study, the diversity and activity of sulfur-metabolizing bacteria were evaluated along a temperature gradient (48-69 °C) in non-acidic phototrophic mats of the Porcelana hot spring (Northern Patagonia, Chile) using complementary meta-omic methodologies and specific amplification of the A (APS reductase) and B (thiosulfohydrolase) genes. Overall, the key players in sulfur metabolism varied mostly in abundance along the temperature gradient, which is relevant for evaluating the possible implications of microorganisms associated with sulfur cycling under the current global climate change scenario. Our results strongly suggest that sulfate reduction occurs throughout the whole temperature gradient, being supported by different taxa depending on temperature. Assimilative sulfate reduction is the most relevant pathway in terms of taxonomic abundance and activity, whereas the sulfur-oxidizing system (Sox) is likely to be more diverse at low rather than at high temperatures. Members of the phylum showed higher sulfur cycle-related transcriptional activity at 66 °C, with a potential contribution to sulfate reduction and oxidation to thiosulfate. In contrast, at the lowest temperature (48 °C), and (both , also known as ) showed a higher contribution to dissimilative sulfate reduction/oxidation as well as to thiosulfate metabolism. and were especially active in assimilatory sulfate reduction. Analysis of the A and B genes pointed to members of the order ( ) as the most dominant and active along the temperature gradient for these genes. Changes in the diversity and activity of different sulfur-metabolizing bacteria in photoautotrophic microbial mats along a temperature gradient revealed their important role in hot spring environments, especially the main primary producers ( / ) and diazotrophs ( ), showing that carbon, nitrogen, and sulfur cycles are highly linked in these extreme systems.