TP53 mutation prevalence in normal airway epithelium as a biomarker for lung cancer risk

Background There is a need for biomarkers that improve accuracy compared with current demographic risk indices to detect individuals at the highest lung cancer risk. Improved risk determination will enable more effective lung cancer screening and better stratification of lung nodules into high or lo...

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Bibliographic Details
Published in:BMC cancer 2023-08, Vol.23 (1), p.1-783, Article 783
Main Authors: Craig, Daniel J, Crawford, Erin L, Chen, Heidi, Grogan, Eric L, Deppen, Steven A, Morrison, Thomas, Antic, Sanja L, Massion, Pierre P, Willey, James C
Format: Article
Language:English
Subjects:
Analysis
Bar codes
Biological markers
Biomarker
Biomarkers
Biopsy
Cancer
Cancer screening
Care and treatment
CT imaging
Design
Diagnosis
Epithelial cells
Epithelium
Exons
Gene mutations
Genetic aspects
Genomes
Genomics
Health aspects
Lung cancer
Lung cancer early detection
Lung cancer prevention
Lung nodule risk classification
Lung nodules
Mutation
Next generation sequencing
p53 Protein
Prevention
Respiratory tract
TP53
Tumor proteins
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Summary:Background There is a need for biomarkers that improve accuracy compared with current demographic risk indices to detect individuals at the highest lung cancer risk. Improved risk determination will enable more effective lung cancer screening and better stratification of lung nodules into high or low-risk category. We previously reported discovery of a biomarker for lung cancer risk characterized by increased prevalence of TP53 somatic mutations in airway epithelial cells (AEC). Here we present results from a validation study in an independent retrospective case-control cohort. Methods Targeted next generation sequencing was used to identify mutations within three TP53 exons spanning 193 base pairs in AEC genomic DNA. Results TP53 mutation prevalence was associated with cancer status (P < 0.001). The lung cancer detection receiver operator characteristic (ROC) area under the curve (AUC) for the TP53 biomarker was 0.845 (95% confidence limits 0.749-0.942). In contrast, TP53 mutation prevalence was not significantly associated with age or smoking pack-years. The combination of TP53 mutation prevalence with PLCO.sub.M2012 risk score had an ROC AUC of 0.916 (0.846-0.986) and this was significantly higher than that for either factor alone (P < 0.03). Conclusions These results support the validity of the TP53 mutation prevalence biomarker and justify taking additional steps to assess this biomarker in AEC specimens from a prospective cohort and in matched nasal brushing specimens as a potential non-invasive surrogate specimen. Keywords: Lung cancer prevention, Lung cancer early detection, Biomarker, Next generation sequencing, TP53, Lung nodule risk classification
ISSN:1471-2407
1471-2407
DOI:10.1186/s12885-023-11266-7