Identification of cytoplasm type and nuclear Ms gene in onion cultivars

• Published in: Pesquisa agropecuária tropical 2024-10, Vol.54, p.e79620
• Main Authors: Daniel Pedrosa Alves, Candida Elisa Manfio, Gustavo Henrique Ferrero Klabunde, Edivânio Rodrigues de Araújo, Fábio Satoshi Higashikawa, Rafael Simões Tomaz, Leonardo Lopes Bhering, Michael John Havey
• Format: Article
• Language: English
• Subjects: allium cepa l; cytoplasmic male sterility; onion breeding
• ISSN: 1983-4063
• DOI: 10.1590/1983-40632024v5479620

Open-pollinated onion cultivars predominate in the southern region of Brazil, due to their higher adaptability to local climatic conditions, unlike commercial hybrids, which have shown a lower adaptability. Cytoplasmic male sterility (CMS) systems are employed to develop hybrid onion cultivars. Two molecular markers, 5’cob and orfA501, were used to differentiate the cytoplasm type, and the AcSKP1 marker to identify the nuclear male fertility-restoring locus (Ms). A total of 1,126 plants from the most common onion cultivars grown in southern Brazil, including Bola Precoce®, Crioula®, Valessul®, Mega®, Joia® and Princesa do Sul®, were analyzed using all the three markers. An extremely rare occurrence of the S cytoplasm was observed among the cultivars, being detected in only 1.8 % of the samples, while the T cytoplasm was the most prevalent, accounting for 56.3 % of the samples. Among the 1,126 plants analyzed, only three exhibited the S cytoplasm and were recessive for the Ms-locus (Smsms). Additionally, 49 plants with the N-cytoplasm (as per the Engelke’s classification) and recessive for the nuclear genotype (Nmsms) were identified, 45 of which were pollen producers. The male-fertility restoration occurred in 22.2 % of the crosses between Tmsms male-sterile plants and male-fertile N cytoplasmic-msms plants (as per the Engelke’s classification).