Over-Expression of βII-Tubulin and Especially Its Localization in Cell Nuclei Correlates with Poorer Outcomes in Colorectal Cancer

Tubulin is a heterodimer of α and β subunits, both existing as isotypes differing in amino acid sequence encoded by different genes. Specific isotypes of tubulin have associations with cancer that are not well understood. Previous studies found that βII-tubulin is expressed in a number of transforme...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2019-01, Vol.8 (1), p.25
Main Authors: Ruksha, Kseniya, Mezheyeuski, Artur, Nerovnya, Alexander, Bich, Tatyana, Tur, Gennady, Gorgun, Julia, Luduena, Richard, Portyanko, Anna
Format: Article
Language:English
Subjects:
Aged
Amino acid sequence
Biomarkers, Tumor - metabolism
Cancer
cancer prognosis
Cancer therapies
Cell Nucleus - metabolism
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - pathology
Cytoplasm
Disease Progression
Female
Humans
Isotypes
Life expectancy
Life span
Localization
Male
Metastases
Metastasis
microtubules
Microtubules - metabolism
Middle Aged
Monoclonal antibodies
nuclear tubulin
Nuclei
Overexpression
Prognosis
Transformed cells
Tubulin
Tubulin - metabolism
Tumors
βII-tubulin
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Summary:Tubulin is a heterodimer of α and β subunits, both existing as isotypes differing in amino acid sequence encoded by different genes. Specific isotypes of tubulin have associations with cancer that are not well understood. Previous studies found that βII-tubulin is expressed in a number of transformed cells and that this isotype is found in cell nuclei in non-microtubule form. The association of βII expression and its nuclear localization with cancer progression has not previously been addressed. We here used a monoclonal antibody to βII to examine patients with colorectal cancer and found that patients whose tumors over-express βII have a greatly decreased life expectancy which is even shorter in those patients with nuclear βII. Our results suggest that βII-tubulin may facilitate cancer growth and metastasis and, to accomplish this, may not need to be in microtubule form. Furthermore, βII expression and localization could be a useful prognostic marker. We also found that βII appears in the nuclei of otherwise normal cells adjacent to the tumor. It is possible therefore that cancer cells expressing βII influence nearby cells to do the same and to localize βII in their nuclei by an as yet uncharacterized regulatory pathway.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells8010025