A myosin II nanomachine mimicking the striated muscle

• Published in: Nature communications 2018-08, Vol.9 (1), p.3532-10, Article 3532
• Main Authors: Pertici, Irene, Bongini, Lorenzo, Melli, Luca, Bianchi, Giulio, Salvi, Luca, Falorsi, Giulia, Squarci, Caterina, Bozó, Tamás, Cojoc, Dan, Kellermayer, Miklós S. Z., Lombardi, Vincenzo, Bianco, Pasquale
• Format: Article
• Language: English
• Subjects: 140/125; 147/3; 631/443; 631/57; 82/83; Actin; Actin Cytoskeleton - metabolism; Actin Cytoskeleton - physiology; Adenosine Triphosphate - metabolism; Animals; ATP; Cardiac muscle; Contraction; Cytoskeleton; Dimers; Experiments; Humanities and Social Sciences; Kinetics; Lasers; Male; Mechanics; Mimicry; Molecular motors; multidisciplinary; Muscle contraction; Muscle Contraction - physiology; Muscle, Skeletal - metabolism; Muscle, Skeletal - physiology; Muscle, Striated - metabolism; Muscle, Striated - physiology; Muscles; Myosin; Myosin Type II - metabolism; Pharmacology; Proteins; Rabbits; Science; Science (multidisciplinary); Skeletal muscle; Velocity
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-018-06073-9

The contraction of striated muscle (skeletal and cardiac muscle) is generated by ATP-dependent interactions between the molecular motor myosin II and the actin filament. The myosin motors are mechanically coupled along the thick filament in a geometry not achievable by single-molecule experiments. Here we show that a synthetic one-dimensional nanomachine, comprising fewer than ten myosin II dimers purified from rabbit psoas, performs isometric and isotonic contractions at 2 mM ATP, delivering a maximum power of 5 aW. The results are explained with a kinetic model fitted to the performance of mammalian skeletal muscle, showing that the condition for the motor coordination that maximises the efficiency in striated muscle is a minimum of 32 myosin heads sharing a common mechanical ground. The nanomachine offers a powerful tool for investigating muscle contractile-protein physiology, pathology and pharmacology without the potentially disturbing effects of the cytoskeletal—and regulatory—protein environment. There is interest in mimicking striated muscle for a range of applications including nanomachines. Here, the authors report on synthetic 1D nanomachines which are used to study an ensemble of myosin motors interacting with an actin filament with potential to create assays of muscle related diseases