Molecular detection and characterization of Anaplasma marginale infecting cattle, buffalo, and camel populations in southern Egypt

Anaplasmosis is a severe tickborne disease of ruminants caused by . is distributed worldwide and attacks erythrocytes, resulting in an increased body temperature, anemia, jaundice, abortion, and, in some cases, death. Animals infected with this pathogen become lifelong carriers. In this study, we ai...

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Bibliographic Details
Published in:Frontiers in veterinary science 2023-05, Vol.10, p.1169323-1169323
Main Authors: Mahmoud, Hassan Y A H, Ali, Alsagher O, Tanaka, Tetsuya
Format: Article
Language:English
Subjects:
Anaplasma marginale
buffalo
camel
cattle
Egypt
PCR
Veterinary Science
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Summary:Anaplasmosis is a severe tickborne disease of ruminants caused by . is distributed worldwide and attacks erythrocytes, resulting in an increased body temperature, anemia, jaundice, abortion, and, in some cases, death. Animals infected with this pathogen become lifelong carriers. In this study, we aimed to detect and characterize isolated from cattle, buffalo, and camel populations using novel molecular techniques in southern Egypt. In total, 250 samples (from 100 cattle, 75 water buffaloes, and 75 camels) were analyzed by PCR for the presence of , specifically . The animals varied in breed, age, and gender, with most showing no signs of severe disease. By species, was found in 61 out of 100 (61%) cattle, 9 out of 75 (12%) buffaloes, and only 5 out of 75 (6.66%) camels. All -positive samples were examined for the heat-shock protein gene and, additionally, for ( ) and ( ) genes to enhance specificity. Phylogenetic analysis of targeted three genes ( , and ). This study provides the first report on using three genes for detection in in southern Egypt and generated new phylogenetic data for infections in camels. is endemic in different animal species in southern Egypt. Screening herds for is recommended even when the signs of anaplasmosis are absent.
ISSN:2297-1769
2297-1769
DOI:10.3389/fvets.2023.1169323