Characterization of Differentially Expressed miRNAs by CXCL12/SDF-1 in Human Bone Marrow Stromal Cells

Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are smal...

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Bibliographic Details
Published in:Biomolecular concepts 2021-10, Vol.12 (1), p.132-143
Main Authors: Potter, Matthew L., Smith, Kathryn, Vyavahare, Sagar, Kumar, Sandeep, Periyasamy-Thandavan, Sudharsan, Hamrick, Mark, Isales, Carlos M., Hill, William D., Fulzele, Sadanand
Format: Article
Language:English
Subjects:
Bioinformatics
Biosynthesis
Bone healing
Bone marrow
bone marrow stromal cells
Cell differentiation
Cell migration
CXCL12 protein
Epigenetics
Fractures
Gene expression
MicroRNAs
miRNA
Mucin
SDF-1
SDF-1 protein
Stem cells
Stromal cells
Thyroid
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Summary:Stromal cell-derived factor 1 (SDF-1) is known to influence bone marrow stromal cell (BMSC) migration, osteogenic differentiation, and fracture healing. We hypothesize that SDF-1 mediates some of its effects on BMSCs through epigenetic regulation, specifically via microRNAs (miRNAs). MiRNAs are small non-coding RNAs that target specific mRNA and prevent their translation. We performed global miRNA analysis and determined several miRNAs were differentially expressed in response to SDF-1 treatment. Gene Expression Omnibus (GEO) dataset analysis showed that these miRNAs play an important role in osteogenic differentiation and fracture healing. KEGG and GO analysis indicated that SDF-1 dependent miRNAs changes affect multiple cellular pathways, including fatty acid biosynthesis, thyroid hormone signaling, and mucin-type O-glycan biosynthesis pathways. Furthermore, bioinformatics analysis showed several miRNAs target genes related to stem cell migration and differentiation. This study's findings indicated that SDF-1 induces some of its effects on BMSCs function through miRNA regulation.
ISSN:1868-5021
1868-5021
1868-503X
DOI:10.1515/bmc-2021-0015