Rapamycin Prevents cyclophosphamide-induced Over-activation of Primordial Follicle pool through PI3K/Akt/mTOR Signaling Pathway in vivo

Primordial follicular depletion has thought to be a common adverse effect of chemotherapy especially for female of reproductive age. The study aimed to evaluate the protective effect of rapamycin on the primordial follicles and its potential mechanism for patients receiving chemotherapy. 8-week old...

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Bibliographic Details
Published in:Journal of ovarian research 2017-08, Vol.10 (1), p.56-56, Article 56
Main Authors: Zhou, Linyan, Xie, Yanqiu, Li, Song, Liang, Yihua, Qiu, Qi, Lin, Haiyan, Zhang, Qingxue
Format: Article
Language:English
Subjects:
Animals
Anti-Mullerian Hormone - blood
Antineoplastic Agents, Alkylating - adverse effects
Cancer
Chemotherapy
Cyclophosphamide
Cyclophosphamide - adverse effects
Dosage and administration
Female
Health aspects
Mice, Inbred BALB C
Ovarian Follicle - drug effects
Ovarian Follicle - pathology
Phosphatidylinositol 3-Kinases - metabolism
PI3K/Akt/mTOR
Primordial follicle
Proteins
Proto-Oncogene Proteins c-akt - metabolism
Rapamycin
Ribosomal Protein S6 - metabolism
Signal Transduction - drug effects
Sirolimus - pharmacology
TOR Serine-Threonine Kinases - antagonists & inhibitors
TOR Serine-Threonine Kinases - metabolism
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Summary:Primordial follicular depletion has thought to be a common adverse effect of chemotherapy especially for female of reproductive age. The study aimed to evaluate the protective effect of rapamycin on the primordial follicles and its potential mechanism for patients receiving chemotherapy. 8-week old BALB/c female mice were randomly assigned into four groups (control; rapamycin; cyclophosphamide; and rapamycin combined with cyclophosphamide). Hematoxylin staining, immunohistochemical, TUNEL, western blotting and ELISA were employed to assess inter-group differences using Student's t-test and Mann-Whitney test. Cyclophosphamide depleted the follicular reserve and induced the phosphorylation of the key proteins of PI3K/Akt/mTOR pathway in mice in a dose-dependent manner. Co-treatment with rapamycin significantly reduced primordial follicle loss at all cyclophosphamide dose groups and prevent the follicle growth wave caused by cyclophosphamide treatment (P 
ISSN:1757-2215
1757-2215
DOI:10.1186/s13048-017-0350-3