Development of a bacterial bioassay for atrazine and cyanuric acid detection

The s-triazine herbicides are compounds which can disseminate into soils and water. Due to their toxic effects on living organisms, their concentrations in drinking water are legislated by WHO recommendations. Here we have developed for the first time, to the best of our knowledge, an alternative me...

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Bibliographic Details
Published in:Frontiers in microbiology 2015-03, Vol.6, p.211
Main Authors: Hua, Anna, Gueuné, Hervé, Cregut, Mickaël, Thouand, Gérald, Durand, Marie-José
Format: Article
Language:English
Subjects:
Analytical chemistry
Bacteria
Bacteriology
bioassay
bioluminescence
biosensor
Biotechnology
Chemical Sciences
Environmental Engineering
Environmental Sciences
Life Sciences
Microbiology
Microbiology and Parasitology
Triazine
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Summary:The s-triazine herbicides are compounds which can disseminate into soils and water. Due to their toxic effects on living organisms, their concentrations in drinking water are legislated by WHO recommendations. Here we have developed for the first time, to the best of our knowledge, an alternative method for physicochemical quantification using two bioluminescent bacterial biosensors: E. coli SM003 for cyanuric acid detection and E. coli SM004 for both atrazine and cyanuric acid detection. The concentration of cyanuric acid detection for E. coli SM003 ranges from 7.83 μM to 2.89 mM, and for E. coli SM004 ranges from 0.22 to 15 μM. Moreover, atrazine detection by E. coli SM004 ranges from 1.08 to 15 μM. According to WHO recommendations, the cyanuric acid detection range is sensitive enough to discriminate between polluted and drinking water. Nevertheless, the detection of atrazine by E. coli SM004 is only applicable for high concentrations of contaminants.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2015.00211