GZMA suppressed GPX4-mediated ferroptosis to improve intestinal mucosal barrier function in inflammatory bowel disease

• Published in: Cell communication and signaling 2024-10, Vol.22 (1), p.474-13, Article 474
• Main Authors: Niu, Rongwei, Lan, Jiaoli, Liang, Danxia, Xiang, Li, Wu, Jiaxin, Zhang, Xiaoyan, Li, Zhiling, Chen, Huan, Geng, Lanlan, Xu, Wanfu, Gong, Sitang, Yang, Min
• Format: Article
• Language: English
• Subjects: Animals; Care and treatment; CDX2; Cell death; Cyclic AMP Response Element-Binding Protein - metabolism; Development and progression; Ferroptosis; GZMA; Health aspects; Humans; IBD; Inflammatory bowel diseases; Inflammatory Bowel Diseases - genetics; Inflammatory Bowel Diseases - metabolism; Inflammatory Bowel Diseases - pathology; Intestinal Barrier Function; Intestinal mucosa; Intestinal Mucosa - metabolism; Mice; Mice, Inbred C57BL; Phospholipid Hydroperoxide Glutathione Peroxidase - genetics; Phospholipid Hydroperoxide Glutathione Peroxidase - metabolism; Proteases
• ISSN: 1478-811X; 1478-811X
• DOI: 10.1186/s12964-024-01836-y

Our previous study has demonstrated a decreased colonic CD8 CD39 T cells, enrichment of granzyme A (GZMA), was found in pediatric-onset colitis and inflammatory bowel disease (IBD) characterized by impaired intestinal barrier function. However, the influence of GZMA on intestinal barrier function remains unknown. Western blotting(WB), real-time PCR (qPCR), immunofluorescence (IF) and in vitro permeability assay combined with intestinal organoid culture were used to detect the effect of GZMA on intestinal epithelial barrier function in vivo and in vitro. Luciferase, immunoprecipitation (IP) and subcellular fractionation isolation were performed to identify the mechanism through which GZMA modulated intestinal epithelial barrier function. Herein, we, for the first time, demonstrated that CD8 CD39 T cells promoted intestinal epithelial barrier function through GZMA, leading to induce Occludin(OCLN) and Zonula Occludens-1(ZO-1) expression, which was attributed to enhanced CDX2-mediated cell differentiation caused by increased glutathione peroxidase 4(GPX4)-induced ferroptosis inhibition in vivo and in vitro. Mechanically, GZMA inhibited intestinal epithelial cellular PDE4B activation to trigger cAMP/PKA/CREB cascade signaling to increase CREB nuclear translocation, initiating GPX4 transactivity. In addition, endogenous PKA interacted with CREB, and this interaction was enhanced in response to GZMA. Most importantly, administration of GZMA could alleviate DSS-induced colitis in vivo. These findings extended the novel insight of GZMA contributed to intestinal epithelial cell differentiation to improve barrier function, and enhacement of GZMA could be a promising strategy to patients with IBD.