The potential of using semitendinosus tendon as autograft in rabbit meniscus reconstruction

• Published in: Scientific reports 2017-08, Vol.7 (1), p.7033-7033, Article 7033
• Main Authors: Li, Chenxi, Hu, Xiaoqing, Meng, Qingyang, Zhang, Xin, Zhu, Jingxian, Dai, Linghui, Cheng, Jin, Zhong, Mingjin, Shi, Weili, Ren, Bo, Zhang, Jiying, Fu, Xin, Duan, Xiaoning, Ao, Yingfang
• Format: Article
• Language: English
• Subjects: 13/100; 13/107; 38/77; 631/532/489; 631/61/54/993; Animals; Autografts; Biocompatibility; Biomaterials; Cartilage; Collagen (type I); Collagen (type II); Degeneration; Hamstring Tendons - surgery; Humanities and Social Sciences; Knee; Mechanical properties; Meniscus; Meniscus - surgery; Mesenchymal Stem Cell Transplantation - methods; Mesenchymal Stem Cells - physiology; Mesenchyme; multidisciplinary; Rabbits; Science; Science (multidisciplinary); Skin & tissue grafts; Stem cells; Surgery; Tissue engineering; Transplantation; Transplantation, Autologous - methods; Treatment Outcome
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-017-07166-z

Since transplantation of meniscal allograft or artificial menisci is limited by graft sources and a series of adverse events, substitution for meniscus reconstruction still needs to be explored. Natural biomaterials, which can provide a unique 3-D microenvironment, remain a promising alternative for tissue engineering. Among them, autograft is a preferred option for its safety and excellent biocompatibility. In this study, we utilized semitendinosus tendon autograft in meniscus reconstruction to investigate its fibrochondrogenic metaplasticity potential and chondroprotective effect. Tendon-derived stem cells (TDSCs) and synovial-derived mesenchymal stem cells (SMSCs), two most important stem cell sources in our strategy, exhibited excellent viability, distribution, proliferation and fibrochondrogenic differentiation ability in decellularized semitendinosus tendon (DST) scaffolds in vitro . Histologic evaluation of the tendon grafts in vivo suggested endogenous stem cells differentiated into fibrochondrocytes, synthesized proteoglycan, type II collagen and radial type I collagen at 12 weeks and 24 weeks post-surgery. As for elastic modulus and hardness of the grafts, there were no significant differences between native meniscus and regenerated meniscus at 24 weeks. The protection of condylar cartilage from degeneration was significantly better in the reconstruction group comparing to control group. Overall, semitendinosus tendon autograft seems to be a promising substitution in meniscus reconstruction.