Neutrophil extracellular traps in the cross-talk between periodontitis and chronic kidney disease

• Published in: BMC oral health 2024-11, Vol.24 (1), p.1357-10, Article 1357
• Main Authors: Hu, Shucheng, Yang, Ruhan, Yang, Wenying, Tang, Jiaqi, Yu, Weijun, Zhao, Dan, Lin, Lu, Gu, Yuting, Jin, Min, Xu, Ziyuan, Wang, Qin, Lu, Eryi
• Format: Article
• Language: English
• Subjects: Adult; Aged; Antibodies; Blood; Cardiovascular disease; Case-Control Studies; Chronic kidney disease; Chronic kidney failure; Clinical medicine; Clinical practice guidelines; Diabetes; DNA - analysis; Extracellular Traps - metabolism; Extrachromosomal DNA; Female; Flow Cytometry; Fluid flow; Gingiva - metabolism; Gingival Crevicular Fluid - chemistry; Gum disease; Health aspects; Humans; Immunofluorescence; Kidney diseases; Leukocytes (neutrophilic); Lupus; Male; Measurement; Middle Aged; Neutrophil extracellular traps; Neutrophils; Pathogens; Periodontal Index; Periodontal therapy; Periodontitis; Periodontitis - complications; Periodontitis - therapy; Renal Insufficiency, Chronic - blood; Renal Insufficiency, Chronic - complications; Renal Insufficiency, Chronic - therapy; Risk factors; Statistical analysis; Systemic diseases; Teeth
• ISSN: 1472-6831; 1472-6831
• DOI: 10.1186/s12903-024-05071-2

The objective was to evaluate the level of neutrophil extracellular traps (NETs) in patients with chronic kidney disease (CKD) and periodontitis, and to explore the relationship between NETs and both diseases. 63 CKD and 40 non-CKD participants were recruited and underwent periodontal examination, among which 35 early CKD patients underwent periodontal therapy. The concentrations of NETs were determined by dsDNA assay in gingival crevicular fluid (GCF) and plasma, and by flow cytometry or immunofluorescence assay in blood and gingival tissues. The correlations between NETs and clinical parameters were analyzed. The influence of periodontal therapy on periodontitis, CKD and NETs concentrations was also evaluated. CKD patients had higher concentrations of NETs in plasma than non-CKD patients, and NETs concentrations were also increased in both GCF and plasma of patients with periodontitis than that of periodontally healthy patients. NETs concentrations were positively correlated with increased clinical parameters of CKD and periodontitis. The positive correlation between CKD and periodontitis was demonstrated. Moreover, periodontal therapy ameliorated periodontitis and CKD, and reduced NETs concentrations in GCF of patients. This study revealed that NETs might be a possible bridge between periodontitis and CKD, and suggested the potential target for therapy.