Characterization of two lipoproteins containing apolipoproteins B and E from lesion-free human aortic intima

Lesion-free areas of aortic intimas from seven men, 30 to 49 years old, were extracted with aqueous buffer within a few hours after an accidental or sudden death. Two lipoprotein fractions could be isolated by density gradient ultracentrifugation from all cases. The mean composition of fraction I (d...

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Bibliographic Details
Published in:Journal of lipid research 1988-05, Vol.29 (5), p.563-572
Main Authors: Ylä-Herttuala, S, Jaakkola, O, Ehnholm, C, Tikkanen, M J, Solakivi, T, Särkioja, T, Nikkari, T
Format: Article
Language:English
Subjects:
Adult
Animals
Antibodies, Monoclonal
Aorta - analysis
Apolipoproteins B - analysis
Apolipoproteins E - analysis
Autopsy
Cells, Cultured
Electrophoresis, Polyacrylamide Gel
Humans
Immunodiffusion
Isoelectric Focusing
Lipoproteins - analysis
Male
Mice
Mice, Inbred Strains
Microscopy, Electron
Middle Aged
Ultracentrifugation - methods
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Summary:Lesion-free areas of aortic intimas from seven men, 30 to 49 years old, were extracted with aqueous buffer within a few hours after an accidental or sudden death. Two lipoprotein fractions could be isolated by density gradient ultracentrifugation from all cases. The mean composition of fraction I (d less than 1.012 g/ml) resembled that reported for the cholesteryl ester-rich, beta-migrating very low density lipoprotein (beta-VLDL); the composition of fraction II (d 1.021-1.046 g/ml) resembled that of plasma low density lipoprotein (LDL). Mean diameter of the particles was 35 +/- 8 nm in fraction I and 25 +/- 5 nm in fraction II (22 +/- 2 nm in plasma LDL). Both fractions contained apolipoproteins B (apoB) and E (apoE), and had increased electrophoretic mobilities and reduced contents of linoleic acid. The immunoreactivity of apoB to a polyclonal and two monoclonal antibodies in both fractions was not different from that of plasma lipoproteins. The apoE isoform patterns in both fractions were similar to those obtained from the respective postmortem plasmas. When incubated with mouse peritoneal macrophages, fractions I and II enhanced the incorporation of radioactive oleate into cholesteryl esters by 10- to 20-fold and 3- to 4-fold, respectively, in comparison to plasma LDL. In conclusion, our results indicate that lesion-free human aortic intima contains two types of apoB- and apoE-containing lipoprotein particles, both of which might be potentially atherogenic.
ISSN:0022-2275
DOI:10.1016/s0022-2275(20)38503-5