Intracellular trafficking of Notch orchestrates temporal dynamics of Notch activity in the fly brain

• Published in: Nature communications 2021-04, Vol.12 (1), p.2083-2083, Article 2083
• Main Authors: Wang, Miaoxing, Han, Xujun, Liu, Chuyan, Takayama, Rie, Yasugi, Tetsuo, Ei, Shin-Ichiro, Nagayama, Masaharu, Tanaka, Yoshitaro, Sato, Makoto
• Format: Article
• Language: English
• Subjects: 14; 14/19; 14/34; 38/1; 38/32; 38/35; 38/89; 631/136/142; 631/136/368/2430; 631/136/532/2182; 631/378/2571/2573; 631/378/2571/2575; 64/24; Animals; Brain; Brain - metabolism; Cell Differentiation; Drosophila; Drosophila melanogaster; Drosophila Proteins - metabolism; Endosomes; Endosomes - metabolism; Fruit flies; Gene Expression Regulation, Developmental; Gene Knockdown Techniques; Humanities and Social Sciences; Insects; Intracellular; Intracellular Signaling Peptides and Proteins - metabolism; multidisciplinary; Neuroblasts; Neurogenesis; Nonlinear systems; Protein Transport - genetics; Protein Transport - physiology; rab GTP-Binding Proteins - genetics; rab4 GTP-Binding Proteins - genetics; Receptors, Notch - metabolism; Science; Science (multidisciplinary); Signal Transduction; Transcription Factors; Wave fronts
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-22442-3

While Delta non-autonomously activates Notch in neighboring cells, it autonomously inactivates Notch through cis -inhibition, the molecular mechanism and biological roles of which remain elusive. The wave of differentiation in the Drosophila brain, the ‘proneural wave’, is an excellent model for studying Notch signaling in vivo. Here, we show that strong nonlinearity in cis -inhibition reproduces the second peak of Notch activity behind the proneural wave in silico. Based on this, we demonstrate that Delta expression induces a quick degradation of Notch in late endosomes and the formation of the twin peaks of Notch activity in vivo. Indeed, the amount of Notch is upregulated and the twin peaks are fused forming a single peak when the function of Delta or late endosomes is compromised. Additionally, we show that the second Notch peak behind the wavefront controls neurogenesis. Thus, intracellular trafficking of Notch orchestrates the temporal dynamics of Notch activity and the temporal patterning of neurogenesis. During Drosophila development, two peaks of Notch activity propagate across the neuroepithelium to generate neuroblasts. Here, the authors show Notch cis -inhibition under the control of intracellular Notch trafficking establishes these two peaks, which temporally control neurogenesis in the brain.