Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis

The loss of germ cells and spermatogenic failure in non-obstructive azoospermia are believed to be the main causes of male infertility. Laboratory studies have used in vitro testicular models and different 3-dimensional (3D) culture systems for preservation, proliferation and differentiation of sper...

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Bibliographic Details
Published in:Stem cell research & therapy 2023-09, Vol.14 (1), p.1-262, Article 262
Main Authors: Salem, Maryam, Khadivi, Farnaz, Javanbakht, Parinaz, Mojaverrostami, Sina, Abbasi, Mehdi, Feizollahi, Narjes, Abbasi, Yasaman, Heidarian, Ehsan, Rezaei Yazdi, Farzane
Format: Article
Language:English
Subjects:
3D culture system
Analysis
Biological products
Biomaterials
Biopsy
Cell culture
Cell differentiation
Cell proliferation
Connective tissue
Cryopreservation
Differentiation
Ethylenediaminetetraacetic acid
Fertility
Germ cells
Growth factors
In vitro spermatogenesis
Infertility
Males
Microenvironments
Organ culture
Preservation
Proliferation
Puberty
Reproductive technologies
Review
Sperm
Spermatogenesis
Spermatogonial stem cells
Stem cells
Testes
Toxicity
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Summary:The loss of germ cells and spermatogenic failure in non-obstructive azoospermia are believed to be the main causes of male infertility. Laboratory studies have used in vitro testicular models and different 3-dimensional (3D) culture systems for preservation, proliferation and differentiation of spermatogonial stem cells (SSCs) in recent decades. The establishment of testis-like structures would facilitate the study of drug and toxicity screening, pathological mechanisms and in vitro differentiation of SSCs which resulted in possible treatment of male infertility. The different culture systems using cellular aggregation with self-assembling capability, the use of different natural and synthetic biomaterials and various methods for scaffold fabrication provided a suitable 3D niche for testicular cells development. Recently, 3D culture models have noticeably used in research for their architectural and functional similarities to native microenvironment. In this review article, we briefly investigated the recent 3D culture systems that provided a suitable platform for male fertility preservation through organ culture of testis fragments, proliferation and differentiation of SSCs.
ISSN:1757-6512
1757-6512
DOI:10.1186/s13287-023-03466-6