Maackia amurensis Rupr. et Maxim.: Supercritical CO2 Extraction and Mass Spectrometric Characterization of Chemical Constituents

Three types of extraction were used to obtain biologically active substances from the heartwood of M. amurensis: supercritical CO2 extraction, maceration with EtOH, and maceration with MeOH. The supercritical extraction method proved to be the most effective type of extraction, giving the highest yi...

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Published in:Molecules (Basel, Switzerland) Switzerland), 2023-02, Vol.28 (5), p.2026
Main Authors: Razgonova, Mayya P., Cherevach, Elena I., Tekutyeva, Lyudmila A., Fedoreyev, Sergey A., Mishchenko, Natalia P., Tarbeeva, Darya V., Demidova, Ekaterina N., Kirilenko, Nikita S., Golokhvast, Kirill
Format: Article
Language:English
Subjects:
bioactive compounds
Biological activity
Carbon dioxide
Chemical compounds
CO2 extraction
Ethanol
Food
Ions
Lipids
Liquid chromatography
Liquid phases
Maackia amurensis
Maceration
Mass spectrometry
Mass spectroscopy
Pharmaceutical industry
Polyphenols
Positive ions
Scientific imaging
Severe acute respiratory syndrome coronavirus 2
Solvents
Supercritical gas extraction
tandem mass spectrometry
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Summary:Three types of extraction were used to obtain biologically active substances from the heartwood of M. amurensis: supercritical CO2 extraction, maceration with EtOH, and maceration with MeOH. The supercritical extraction method proved to be the most effective type of extraction, giving the highest yield of biologically active substances. Several experimental conditions were investigated in the pressure range of 50–400 bar, with 2% of ethanol as co-solvent in the liquid phase at a temperature in the range of 31–70 °C. The most effective extraction conditions are: pressure of 100 bar and a temperature of 55 °C for M. amurensis heartwood. The heartwood of M. amurensis contains various polyphenolic compounds and compounds of other chemical groups with valuable biological activity. Tandem mass spectrometry (HPLC-ESI—ion trap) was applied to detect target analytes. High-accuracy mass spectrometric data were recorded on an ion trap equipped with an ESI source in the modes of negative and positive ions. The four-stage ion separation mode was implemented. Sixty-six different biologically active components have been identified in M. amurensis extracts. Twenty-two polyphenols were identified for the first time in the genus Maackia.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28052026