Microbial hydrogen consumption leads to a significant pH increase under high-saline-conditions: implications for hydrogen storage in salt caverns

• Published in: Scientific reports 2023-06, Vol.13 (1), p.10564-10564, Article 10564
• Main Authors: Dopffel, Nicole, Mayers, Kyle, Kedir, Abduljelil, Alagic, Edin, An-Stepec, Biwen Annie, Djurhuus, Ketil, Boldt, Daniel, Beeder, Janiche, Hoth, Silvan
• Format: Article
• Language: English
• Subjects: 631/326/171; 631/326/47; 704/172; Bicarbonates; Brines; Caverns; Consumption; Humanities and Social Sciences; Hydrogen; Hydrogen sulfide; Microorganisms; multidisciplinary; Natural gas; pH effects; Salt; Salts; Science; Science (multidisciplinary); Sulfate reduction; Sulfate-reducing bacteria; Sulfates; Uranium
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-37630-y

Salt caverns have been successfully used for natural gas storage globally since the 1940s and are now under consideration for hydrogen (H 2 ) storage, which is needed in large quantities to decarbonize the economy to finally reach a net zero by 2050. Salt caverns are not sterile and H 2 is a ubiquitous electron donor for microorganisms. This could entail that the injected H 2 will be microbially consumed, leading to a volumetric loss and potential production of toxic H 2 S. However, the extent and rates of this microbial H 2 consumption under high-saline cavern conditions are not yet understood. To investigate microbial consumption rates, we cultured the halophilic sulphate-reducing bacteria Desulfohalobium retbaense and the halophilic methanogen Methanocalculus halotolerans under different H 2 partial pressures. Both strains consumed H 2 , but consumption rates slowed down significantly over time. The activity loss correlated with a significant pH increase (up to pH 9) in the media due to intense proton- and bicarbonate consumption. In the case of sulphate reduction, this pH increase led to dissolution of all produced H 2 S in the liquid phase. We compared these observations to a brine retrieved from a salt cavern located in Northern Germany, which was then incubated with 100% H 2 over several months. We again observed a H 2 loss (up to 12%) with a concurrent increase in pH of up to 8.5 especially when additional nutrients were added to the brine. Our results clearly show that sulphate-reducing microbes present in salt caverns consume H 2 , which will be accompanied by a significant pH increase, resulting in reduced activity over time. This potentially self-limiting process of pH increase during sulphate-reduction will be advantageous for H 2 storage in low-buffering environments like salt caverns.