Fabrication and intermolecular interactions of silk fibroin/hydroxybutyl chitosan blended nanofibers

The native extracellular matrix (ECM) is composed of a cross-linked porous network of multifibril collagens and glycosaminoglycans. Nanofibrous scaffolds of silk fibroin (SF) and hydroxybutyl chitosan (HBC) blends were fabricated using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and trifluoroacetic aci...

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Bibliographic Details
Published in:International journal of molecular sciences 2011-04, Vol.12 (4), p.2187-2199
Main Authors: Zhang, Kui-Hua, Yu, Qiao-Zhen, Mo, Xiu-Mei
Format: Article
Language:English
Subjects:
Biocompatibility
Biomedical materials
Chitosan - chemistry
electrospinning
Extracellular matrix
Fibroins - chemistry
Fourier transforms
Hydrogen Bonding
Hydrogen bonds
Morphology
Nanofibers - chemistry
nanofibrous scaffolds
NMR
Nuclear magnetic resonance
Polymer blends
Protein Structure, Secondary
Ratios
SF/HBC blends
Silk - chemistry
Temperature
Thermogravimetry
Tissue Engineering
X-Ray Diffraction
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Summary:The native extracellular matrix (ECM) is composed of a cross-linked porous network of multifibril collagens and glycosaminoglycans. Nanofibrous scaffolds of silk fibroin (SF) and hydroxybutyl chitosan (HBC) blends were fabricated using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and trifluoroacetic acid (TFA) as solvents to biomimic the native ECM via electrospinning. Scanning electronic microscope (SEM) showed that relatively uniform nanofibers could be obtained when 12% SF was blended with 6% HBC at the weight ratio of 50:50. Meanwhile, the average nanofibrous diameter increased when the content of HBC in SF/HBC blends was raised from 20% to 100%. Fourier transform infrared spectra (FTIR) and (13)C nuclear magnetic resonance (NMR) showed SF and HBC molecules existed in hydrogen bonding interactions but HBC did not induce conformation of SF transforming from random coil form to β-sheet structure. X-ray diffraction (XRD) confirmed the different structure of SF/HBC blended nanofibers from both SF and HBC. Thermogravimetry-Differential thermogravimetry (TG-DTG) results demonstrated that the thermal stability of SF/HBC blend nanofibrous scaffolds was improved. The results indicated that the rearrangement of HBC and SF molecular chain formed a new structure due to stronger hydrogen bonding between SF and HBC. These electrospun SF/HBC blended nanofibers may provide an ideal tissue engineering scaffold and wound dressing.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms12042187