First Molecular Identification of Caligus clemensi on Cultured Crimson Snapper Lutjanus erythropterus on Jerejak Island, Penang, Peninsular Malaysia

Fish parasites such as are a serious concern for cultured fish in many regions of the world, including Malaysia. This study was designed to elucidate the parasites' prevalence and intensity coupled with the morphology and molecular identification of on cultured in Jerejak Island, Penang, Penins...

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Bibliographic Details
Published in:Pathogens (Basel) 2022-01, Vol.11 (2), p.188
Main Authors: Yahaya, Zary Shariman, Azizah, Mohd Nor Siti, Alkazmi, Luay, Ravi, Rajiv, Awosolu, Oluwaseun Bunmi
Format: Article
Language:English
Subjects:
Aquaculture
aquaculture industry
Caligus clemensi
Emission analysis
Field emission microscopy
Fish
Fish parasites
Gene sequencing
Genetic testing
Identification
Lutjanus erythropterus
morphological identification
Morphology
Nucleotide sequence
Nucleotides
Parasites
Parasitic diseases
phylogenetic tree
Phylogeny
rRNA 18S
Scanning electron microscopy
Statistical analysis
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Summary:Fish parasites such as are a serious concern for cultured fish in many regions of the world, including Malaysia. This study was designed to elucidate the parasites' prevalence and intensity coupled with the morphology and molecular identification of on cultured in Jerejak Island, Penang, Peninsular Malaysia. The study was carried out on 200 fish specimens of cultured obtained from the GST group aquaculture farm. Parasites were collected from the infested part of fish, and their prevalence and intensity were determined. The parasites were identified morphologically using a field emission scanning electron microscope. Molecular studies were performed through PCR amplification and sequencing. MEGA 5 was used to construct a phylogenetic tree using the pairwise distance method. The results showed that only the parasite was found prevalent on fish with a prevalence and mean intensity (S.D) of 198 (99%) and 36.4 ± 12.2, respectively. The prevalence varied significantly with respect to fish length ( < 0.05). The nucleotide BLAST sequence for 18S ribosomal RNA partial sequences showed 97% with 100% query similarity, E-value 0 with with the accession number DQ123833.1. Conclusively, remains a major parasite of in the study area.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens11020188