Screening of molecular virulence markers in Staphylococcus aureus and Pseudomonas aeruginosa strains isolated from clinical infections

Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts. The frequent emergence of antibiotic-resistant S. aureus and Ps. aeruginosa strains has...

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Bibliographic Details
Published in:International journal of molecular sciences 2010-12, Vol.11 (12), p.5273-5291
Main Authors: Cotar, Ani-Ioana, Chifiriuc, Mariana-Carmen, Dinu, Sorin, Bucur, Marcela, Iordache, Carmen, Banu, Otilia, Dracea, Olguta, Larion, Cristina, Lazar, Veronica
Format: Article
Language:English
Subjects:
Bacteria
Cystic fibrosis
Electronic mail systems
Female
Humans
Iatrogenic Disease
invasive infections
Male
molecular markers
Mortality
Nosocomial infections
Pathogenesis
Pathogens
Polymerase Chain Reaction
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - isolation & purification
Pseudomonas Infections - genetics
Staphylococcal Infections - genetics
Staphylococcus aureus
Staphylococcus aureus - genetics
Staphylococcus aureus - isolation & purification
Staphylococcus infections
Toxins
Virulence
Virulence Factors - genetics
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Summary:Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts. The frequent emergence of antibiotic-resistant S. aureus and Ps. aeruginosa strains has determined the development of new strategies in order to elucidate the different mechanisms used by these bacteria at different stages of the infectious process, providing the scientists with new procedures for preventing, or at least improving, the control of S. aureus and Ps. aeruginosa infections. The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens. We used multiplex and uniplex PCR assays to detect the genes encoding different cell-wall associated and extracellular virulence factors, in order to evaluate potential associations between the presence of putative virulence genes and the outcome of infections caused by these bacteria. Our results demonstrate that all the studied S. aureus and Ps. aeruginosa strains synthesize the majority of the investigated virulence determinants, probably responsible for different types of infections.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms11125273