Effects of arecoline on cell growth, migration, and differentiation in cementoblasts

Abstract Background/purpose Studies have supported a higher prevalence of periodontal disease among areca quid chewers than non-chewers. However, few studies have stated the effects of areca quid on periodontal tissues. The aim of this study was to investigate the inhibitory effects of arecoline, th...

Full description

Saved in:
Bibliographic Details
Published in:Journal of dental sciences 2015-12, Vol.10 (4), p.388-393
Main Authors: Chen, Yi-Juai, Lee, Shiuan-Shinn, Huang, Fu-Mei, Yu, Hui-Chieh, Tsai, Chi-Cheng, Chang, Yu-Chao
Format: Article
Language:English
Subjects:
Advanced Basic Science
arecoline
cementoblasts
cytotoxicity
Dentistry
differentiation
migration
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Background/purpose Studies have supported a higher prevalence of periodontal disease among areca quid chewers than non-chewers. However, few studies have stated the effects of areca quid on periodontal tissues. The aim of this study was to investigate the inhibitory effects of arecoline, the major alkaloid of areca nut, on murine immortalized cementoblast cell line (OCCM.30). Materials and methods Cytotoxicity was judged using tetrazolium bromide reduction assay. Cell migration was evaluated by Transwell assay. In vitro mineral nodule formation was assayed by von Kossa staining. Cell differentiation was examined by alkaline phosphatase activity with substrate assay. The production of osteoprotegerin was evaluated using enzyme-linked immunosorbent assay. Results Arecoline demonstrated cytotoxicity to cementoblasts in a dose-dependent and time-dependent manner (P 
ISSN:1991-7902
DOI:10.1016/j.jds.2015.04.002