Insecticide resistance intensity and efficacy of synergists with pyrethroids in Anopheles gambiae (Diptera: Culicidae) from Southern Togo

Background This study was designed to provide insecticide resistance data for decision-making in terms of resistance management plans in Togo. Methods The susceptibility status of Anopheles gambiae sensu lato (s.l.) to insecticides used in public health was assessed using the WHO tube test protocol....

Full description

Saved in:
Bibliographic Details
Published in:Malaria journal 2022-11, Vol.21 (1), p.1-353, Article 353
Main Authors: Apetogbo, Yawo, Ahadji-Dabla, Koffi M, Soma, Dieudonné Diloma, Amoudji, Adjovi D, Koffi, Edoh, Akagankou, Kossivi I, Bamogo, Rabila, Ngaffo, Kelly Lionelle, Maiga, Samina, Atcha-Oubou, Rachid T, Dorkenoo, Ameyo M, Vizcaino, Lucrecia, Lenhart, Audrey, Diabaté, Abdoulaye, Dabiré, Roch Kounbobr, Ketoh, Guillaume Koffivi
Format: Article
Language:English
Subjects:
Anopheles gambiae
Bio-assays
Bioassays
Decision making
Detoxification
Enzymes
Esterases
Gene frequency
Genotyping
Glutathione
Human diseases
Insecticide resistance
Insecticides
Intensity resistance
Local population
Malaria
Medical laboratories
Methods
Mortality
Mosquitoes
Mutation
Nucleotide sequence
Pesticide resistance
Piperonyl butoxide
Prevention
Public health
Pyrethroids
Risk factors
Software
Togo
Transferases
Vector control
Vector-borne diseases
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background This study was designed to provide insecticide resistance data for decision-making in terms of resistance management plans in Togo. Methods The susceptibility status of Anopheles gambiae sensu lato (s.l.) to insecticides used in public health was assessed using the WHO tube test protocol. Pyrethroid resistance intensity bioassays were performed following the CDC bottle test protocol. The activity of detoxification enzymes was tested using the synergists piperonyl butoxide, S.S.S-tributlyphosphorotrithioate and ethacrinic acid. Species-specific identification of An. gambiae s.l. and kdr mutation genotyping were performed using PCR techniques. Results Local populations of An. gambiae s.l. showed full susceptibility to pirimiphos methyl at Lomé, Kovié, Anié, and Kpèlè Toutou. At Baguida, mortality was 90%, indicating possible resistance to pirimiphos methyl. Resistance was recorded to DDT, bendiocarb, and propoxur at all sites. A high intensity of pyrethroid resistance was recorded and the detoxification enzymes contributing to resistance were oxidases, esterases, and glutathione-s-transferases based on the synergist tests. Anopheles gambiae sensu stricto (s.s.) and Anopheles coluzzii were the main species identified. High kdr L1014F and low kdr L1014S allele frequencies were detected at all localities. Conclusion This study suggests the need to reinforce current insecticide-based malaria control interventions (IRS and LLINs) with complementary tools. Keywords: Malaria, Anopheles gambiae, Vector control, Intensity resistance, Togo
ISSN:1475-2875
1475-2875
DOI:10.1186/s12936-022-04377-2