The SIX5 Protein in Fusarium oxysporum f. sp. cepae Acts as an Avirulence Effector toward Shallot ( Allium cepa L. Aggregatum Group)

f. sp. ( ) causes basal rot disease in species, including onions ( L.) and shallots ( L. Aggregatum group). Among species, shallots can be crossbred with onions and are relatively more resistant to than onions. Thus, shallots are considered a potential disease-resistant resource for onions. However,...

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Bibliographic Details
Published in:Microorganisms (Basel) 2023-11, Vol.11 (12), p.2861
Main Authors: Sakane, Kosei, Kunimoto, Masaaki, Furumoto, Kazuki, Shigyo, Masayoshi, Sasaki, Kazunori, Ito, Shin-Ichi
Format: Article
Language:English
Subjects:
Allium
Allium cepa
Amino acid substitution
Amino acids
Basal rot
Complementation
Fusarium oxysporum
Fusarium oxysporum f. sp. cepae
Genes
Genetic engineering
Leaves
Molecular interactions
Mutants
Onions
Pathogens
Peptides
Polymerase chain reaction
Proteins
shallot
Signs and symptoms
SIX gene family
SIX5
Tomatoes
Vegetables
Virulence
virulence effector
Wilt
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Summary:f. sp. ( ) causes basal rot disease in species, including onions ( L.) and shallots ( L. Aggregatum group). Among species, shallots can be crossbred with onions and are relatively more resistant to than onions. Thus, shallots are considered a potential disease-resistant resource for onions. However, the mechanisms underlying the molecular interactions between shallots and remain unclear. This study demonstrated that SIX5, an effector derived from (FocSIX5), acts as an avirulence effector in shallots. We achieved this by generating a gene knockout mutant in for which experiments which revealed that it caused more severe wilt symptoms in -resistant shallots than the wild-type and gene complementation mutants. Moreover, we demonstrated that a single amino acid substitution (R67K) in FocSIX5 was insufficient to overcome shallot resistance to .
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms11122861