Replacement of dietary saturated FAs by PUFAs in diet and reverse cholesterol transport

• Published in: Journal of lipid research 2008-11, Vol.49 (11), p.2414-2418
• Main Authors: Kralova Lesna, I., Suchanek, P., Kovar, J., Stavek, P., Poledne, R.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Biological Transport, Active - physiology; Cell Line, Tumor; Cholesterol - metabolism; cholesterol efflux; Cross-Over Studies; Diet; Dietary Fats - metabolism; fatty acids; Fatty Acids, Unsaturated - metabolism; Humans; macrophages; Male; Middle Aged; Young Adult
• ISSN: 0022-2275; 1539-7262
• DOI: 10.1194/jlr.M800271-JLR200

Dietary intervention is the first and usually successful approach in the treatment of high LDL cholesterol (LDL-C) concentration, but it is frequently accompanied by a decrease in HDL concentration. We studied 14 male volunteers on two different diets, high saturated fatty acid (SFA) and high PUFA, in a crossover design to test whether a decrease in HDL can affect reverse cholesterol transport from relabeled macrophages. A significant decrease of LDL-C (in mmol/l) after a PUFA diet compared with an SFA diet from 3.15 ± 0.65 to 2.80 ± 0.56 (P < 0.01) was accompanied by a significant decrease of HDL cholesterol (HDL-C) (in mmol/l) from 1.21 ± 0.30 to 1.10 ± 0.32 (P < 0.05). These changes did not affect cholesterol efflux (CHE) from macrophages (9.74 ± 1.46% vs. 9.53 ± 1.41%). There was no correlation between individual changes of HDL-C and changes of CHE. It is concluded that the decrease of HDL-C after successful dietary intervention of LDL-C is not accompanied by a decrease of CHE.