Adaptive Evolution for the Efficient Production of High-Quality d-Lactic Acid Using Engineered Klebsiella pneumoniae

d-Lactic acid serves as a pivotal platform chemical in the production of poly d-lactic acid (PDLA) and other value-added products. This compound can be synthesized by certain bacteria, including . However, industrial-scale lactic acid production in faces challenges due to growth inhibition caused by...

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Bibliographic Details
Published in:Microorganisms (Basel) 2024-06, Vol.12 (6), p.1167
Main Authors: Jiang, Bo, Liu, Jiezheng, Wang, Jingnan, Zhao, Guang, Zhao, Zhe
Format: Article
Language:English
Subjects:
Acid production
Acid resistance
adaptive evolution
Bacterial pneumonia
Biopolymers
Bioprocessing
Cell growth
d -lactic acid
E coli
Evolution & development
Fermentation
Fine chemicals
Food additives
Gene sequencing
Genetic engineering
Genomes
Glucose
Glucose-1-phosphate
Glycerol
Hydrochloric acid
Industrial applications
Klebsiella
Klebsiella pneumoniae
Lactic acid
Metabolism
Microorganisms
organic acid tolerance
Plasmids
Pneumonia
stress response
UTP-glucose-1-phosphate uridylyltransferase
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Summary:d-Lactic acid serves as a pivotal platform chemical in the production of poly d-lactic acid (PDLA) and other value-added products. This compound can be synthesized by certain bacteria, including . However, industrial-scale lactic acid production in faces challenges due to growth inhibition caused by lactic acid stress, which acts as a bottleneck in commercial microbial fermentation processes. To address this, we employed a combination of evolutionary and genetic engineering approaches to create an improved strain with enhanced lactic acid tolerance and production. In flask fermentation experiments, the engineered strain achieved an impressive accumulation of 19.56 g/L d-lactic acid, representing the highest production yield observed in to date. Consequently, this strain holds significant promise for applications in industrial bioprocessing. Notably, our genome sequencing and experimental analyses revealed a novel correlation between UTP-glucose-1-phosphate uridylyltransferase GalU and lactic acid resistance in . Further research is warranted to explore the potential of targeting GalU for enhancing d-lactic acid production.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms12061167