The Family Keeps on Growing: Four Novel Fungal OYEs Characterized

• Published in: International journal of molecular sciences 2022-03, Vol.23 (6), p.3050
• Main Authors: Robescu, Marina Simona, Loprete, Giovanni, Gasparotto, Matteo, Vascon, Filippo, Filippini, Francesco, Cendron, Laura, Bergantino, Elisabetta
• Format: Article
• Language: English
• Subjects: Ascomycota - metabolism; Aspergillus niger; Binding sites; biocatalysis; Biocatalysts; Botryotinia fuckeliana; Catalytic Domain; Crystal structure; E coli; Enzymes; Fungi; Genomes; Homology; NADPH Dehydrogenase - metabolism; old yellow enzyme; Pharmaceutical industry; Proteins; Substrate Specificity; Thermal stability
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23063050

Aiming at expanding the portfolio of Old Yellow Enzymes (OYEs), which have been systematically studied to be employed in the chemical and pharmaceutical industries as useful biocatalysts, we decided to explore the immense reservoir of filamentous fungi. We drew from the genome of the two Ascomycetes and four new members of the OYE superfamily belonging to the classical and thermophilic-like subfamilies. The two OYEs show wider substrate spectra than the OYE homologues, which appear as more specialized biocatalysts. According to their mesophilic origins, the new enzymes neither show high thermostability nor extreme pH optimums. The crystal structures of OYE4 and OYE8 have been determined, revealing the conserved features of the thermophilic-like subclass as well as unique properties, such as a peculiar N-terminal loop involved in dimer surface interactions. For the classical representatives OYE1 and OYE2, model structures were built and analyzed, showing surprisingly wide open access to the active site cavities due to a shorter β6-loop and a disordered capping subdomain.