Genome-wide transcriptional profiling and functional analysis of long noncoding RNAs and mRNAs in chicken macrophages associated with the infection of avian pathogenic E. coli

• Published in: BMC veterinary research 2024-02, Vol.20 (1), p.49-49, Article 49
• Main Authors: Sun, Hongyan, Cao, Xinqi, Sumayya, Ma, Yuyi, Li, Huan, Han, Wei, Qu, Lujiang
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Antibiotics; Apoptosis; Avian pathogenic E. coli; CD86; CD86 antigen; Chickens; Chickens - genetics; Chickens - microbiology; Colibacillosis; E coli; Escherichia coli - genetics; Escherichia coli Infections - microbiology; Escherichia coli Infections - veterinary; Evaluation; Genes; Genetic aspects; Genome-wide association studies; Genomes; Health aspects; Homeostasis; Immune and inflammatory response; Infections; Inflammation; lncRNA TCONS_00007391; lncRNA-mRNA; Macrophages; Non-coding RNA; Phosphatidylinositol; Poultry; Poultry Diseases - genetics; Poultry Diseases - microbiology; RNA, Long Noncoding - genetics; RNA, Messenger - genetics; RNAseq; Signal transduction; Small intestine; Spleen; Therapeutic targets; Toll-like receptors; Vascular endothelial growth factor
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-024-03890-7

Avian pathogenic E. coli (APEC) can cause localized or systemic infections, collectively known as avian colibacillosis, resulting in huge economic losses to poultry industry globally per year. In addition, increasing evidence indicates that long non-coding RNAs (lncRNAs) play a critical role in regulating host inflammation in response to bacterial infection. However, the role of lncRNAs in the host response to APEC infection remains unclear. Here, we found 816 differentially expressed (DE) lncRNAs and 1,798 DE mRNAs in APEC infected chicken macrophages by RNAseq. The identified DE lncRNA-mRNAs were involved in Toll like receptor signaling pathway, VEGF signaling pathway, fatty acid metabolism, phosphatidylinositol signaling system, and other types of O-glycan biosynthesis. Furthermore, we found the novel lncRNA TCONS_00007391 as an important immune regulator in APEC infection was able to regulate the inflammatory response by directly targeting CD86. These findings provided a better understanding of host response to APEC infection and also offered the potential drug targets for therapy development against APEC infection.