Livestock-associated methicillin-resistant Staphylococcus aureus sequence type 398 in humans, Canada

Rates of colonization with livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) sequence type 398 have been high for pigs and pig farmers in Canada, but prevalence rates for the general human population are unknown. In this study, 5 LA-MRSA isolates, 4 of which were obtained from...

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Bibliographic Details
Published in:Emerging infectious diseases 2010-04, Vol.16 (4), p.587-594
Main Authors: Golding, George R, Bryden, Louis, Levett, Paul N, McDonald, Ryan R, Wong, Alice, Wylie, John, Graham, Morag R, Tyler, Shaun, Van Domselaar, Gary, Simor, Andrew E, Gravel, Denise, Mulvey, Michael R
Format: Article
Language:English
Subjects:
Aged
Analysis
Animals
Animals, Domestic - virology
Bacterial Toxins - genetics
CRISPR
Deoxyribonucleases, Type II Site-Specific - genetics
DNA
Drug resistance in microorganisms
Enzymes
Exotoxins - genetics
Female
Genes, Bacterial - genetics
Health aspects
Humans
Infection
Inverted Repeat Sequences - genetics
Leukocidins - genetics
livestock associated
Male
Manitoba - epidemiology
Medical research
Medicine, Experimental
Methicillin-resistant Staphylococcus aureus
Methicillin-Resistant Staphylococcus aureus - genetics
Methicillin-Resistant Staphylococcus aureus - isolation & purification
Middle Aged
Mutagenesis, Insertional - genetics
Pork industry
Prevalence
Saskatchewan - epidemiology
SCCmec
ST398
Staphylococcal infections
Staphylococcal Infections - epidemiology
Staphylococcal Infections - microbiology
Staphylococcus aureus
Swine - microbiology
zoonoses
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Summary:Rates of colonization with livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) sequence type 398 have been high for pigs and pig farmers in Canada, but prevalence rates for the general human population are unknown. In this study, 5 LA-MRSA isolates, 4 of which were obtained from skin and soft tissue infections, were identified from 3,687 tested MRSA isolates from persons in Manitoba and Saskatchewan, Canada. Further molecular characterization determined that these isolates all contained staphylococcal cassette chromosome (SCC) mecV, were negative for Panton-Valentine leukocidin, and were closely related by macrorestriction analysis with the restriction enzyme Cfr91. The complete DNA sequence of the SCCmec region from the isolate showed a novel subtype of SCCmecV harboring clustered regularly interspaced short palindromic repeats and associated genes. Although prevalence of livestock-associated MRSA seems to be low for the general population in Canada, recent emergence of infections resulting from this strain is of public health concern.
ISSN:1080-6040
1080-6059
DOI:10.3201/eid1604.091435