Mussel-inspired human gelatin nanocoating for creating biologically adhesive surfaces

Recombinant human gelatin was conjugated with dopamine using carbodiimide as a surface modifier. This dopamine-coupled human gelatin (D-rhG) was characterized by (1)H-nuclear magnetic resonance, mass spectroscopy, and circular dichroism. D-rhG-coated surface properties were analyzed by physicochemic...

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Bibliographic Details
Published in:International journal of nanomedicine 2014-01, Vol.9 (Issue 1), p.2753-2765
Main Authors: Yang, Xi, Zhu, Liping, Tada, Seiichi, Zhou, Di, Kitajima, Takashi, Isoshima, Takashi, Yoshida, Yasuhiro, Nakamura, Mariko, Yan, Weiqun, Ito, Yoshihiro
Format: Article
Language:English
Subjects:
Adhesives
Adhesives - chemical synthesis
Adhesives - pharmacology
Animals
Bioengineering
Biomedical materials
Biomimetic Materials - chemistry
Bivalvia - chemistry
Cell adhesion
Cell Adhesion - drug effects
Cell Adhesion - physiology
cell culture
Cells, Cultured
Collagen
Contact angle
Dopamine
Dopamine - chemistry
Dopamine - pharmacology
Endothelial Cells - drug effects
Endothelial Cells - physiology
Gelatin
Gelatin - chemistry
Gelatin - pharmacology
Humans
Materials Testing
natural catechols
Original Research
Physiological aspects
Proteins
recombinant human gelatin
Tissue engineering
Titanium
Vascular endothelial growth factor
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Description
Summary:Recombinant human gelatin was conjugated with dopamine using carbodiimide as a surface modifier. This dopamine-coupled human gelatin (D-rhG) was characterized by (1)H-nuclear magnetic resonance, mass spectroscopy, and circular dichroism. D-rhG-coated surface properties were analyzed by physicochemical methods. Additionally, cell attachment and growth on the modified surfaces was assessed using human umbilical endothelial cells. Binding of gelatin onto titanium was significantly enhanced by dopamine conjugation. The thickness of the D-rhG coating depended on the treatment pH; thicker layers were formed at higher pH values, with a maximum thickness of 30 nm. D-rhG enhanced the binding of collagen-binding vascular endothelial growth factor and cell adhesion as compared with gelatin alone, even at the same surface concentration. The D-rhG surface modifier enhanced substrate binding by creating an adhesive nanointerface that increased specific protein binding and cell attachment.
ISSN:1178-2013
1176-9114
1178-2013
DOI:10.2147/ijn.s60624