In vitro comparison of epidural bacteria filters permeability and screening scanning electron microscopy

• Published in: Brazilian journal of anesthesiology (Elsevier) 2015-11, Vol.65 (6), p.491-496
• Main Authors: Sener, Aysin, Erkin, Yuksel, Sener, Alper, Tasdogen, Aydin, Dokumaci, Esra, Elar, Zahide
• Format: Article
• Language: English
• Subjects: Analgesia, Epidural - instrumentation; ANESTHESIOLOGY; Bacteria - isolation & purification; Bacteria filter; Bacterial Adhesion; Filtration - instrumentation; Filtro antibacteriano; Microscopy, Electron, Scanning; Microscópio eletrônico de varredura; Permeability; Pseudomonas aeruginosa; Scanning electron microscope; Staphylococcus aureus; Sterilization
• ISSN: 0104-0014; 1806-907X; 0104-0014
• DOI: 10.1016/j.bjane.2013.08.004

Epidural catheter bacteria filters are barriers in the patient-controlled analgesia/anaesthesia for preventing contamination at the epidural insertion site. The efficiency of these filters varies according to pore sizes and materials. The bacterial adhesion capability of the two filters was measured in vitro experiment. Adhesion capacities for standard Staphylococcus aureus (ATCC 25923) and Pseudomonas aeruginosa (ATCC 27853) strains of the two different filters (Portex and Rusch) which have the same pore size were examined. Bacterial suspension of 0.5 Mc Farland was placed in the patient-controlled analgesia pump, was filtered at a speed of 5mL/h. in continuous infusion for 48h and accumulated in bottle. The two filters were compared with colony counts of bacteria in the filters and bottles. At the same time, the filters and adhered bacteria were monitored by scanning electron microscope. Electron microscopic examination of filters showed that the Portex filter had a granular and the Rusch filter fibrillary structure. Colony counting from the catheter and bottle showed that both of the filters have significant bacterial adhesion capability (p