Roles for the RNA-Binding Protein Caper in Reproductive Output in Drosophila melanogaster

RNA binding proteins (RBPs) play a fundamental role in the post-transcriptional regulation of gene expression within the germline and nervous system. This is underscored by the prevalence of mutations within RBP-encoding genes being implicated in infertility and neurological disease. We previously d...

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Bibliographic Details
Published in:Journal of developmental biology 2022-12, Vol.11 (1), p.2
Main Authors: Tixtha, Erika J, Super, Meg K, Titus, M Brandon, Bono, Jeremy M, Olesnicky, Eugenia C
Format: Article
Language:English
Subjects:
Animal reproduction
Antibodies
Caper
Embryogenesis
Embryos
Experiments
Fertility
Follicles
Fruit juices
Gene expression
Gene regulation
Infertility
Insects
Lethality
Localization
Mating behavior
Morphogenesis
Mutation
Nervous system
Neurological diseases
Oogenesis
Ovaries
Phenotypes
Post-transcription
reproductive output
RNA-binding protein
RNA-binding proteins
RNA-mediated interference
Roles
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Summary:RNA binding proteins (RBPs) play a fundamental role in the post-transcriptional regulation of gene expression within the germline and nervous system. This is underscored by the prevalence of mutations within RBP-encoding genes being implicated in infertility and neurological disease. We previously described roles for the highly conserved RBP Caper in neurite morphogenesis in the larval peripheral system and in locomotor behavior. However, function has not been investigated outside the nervous system, although it is widely expressed in many different tissue types during embryogenesis. Here, we describe novel roles for Caper in fertility and mating behavior. We find that Caper is expressed in ovarian follicles throughout oogenesis but is dispensable for proper patterning of the egg chamber. Additionally, reduced function, through either a genetic lesion or RNA interference-mediated knockdown of in the female germline, results in females laying significantly fewer eggs than their control counterparts. Moreover, this phenotype is exacerbated with age. dysfunction also results in partial embryonic and larval lethality. Given that is highly conserved across metazoa, these findings may also be relevant to vertebrates.
ISSN:2221-3759
2221-3759
DOI:10.3390/jdb11010002