Structural basis for receptor recognition of pollen tube attraction peptides

Transportation of the immobile sperms directed by pollen tubes to the ovule-enclosed female gametophytes is important for plant sexual reproduction. The defensin-like (DEFL) cysteine-rich peptides (CRPs) LUREs play an essential role in pollen tube attraction to the ovule, though their receptors stil...

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Bibliographic Details
Published in:Nature communications 2017-11, Vol.8 (1), p.1331-9, Article 1331
Main Authors: Zhang, Xiaoxiao, Liu, Weijia, Nagae, Takuya T., Takeuchi, Hidenori, Zhang, Heqiao, Han, Zhifu, Higashiyama, Tetsuya, Chai, Jijie
Format: Article
Language:English
Subjects:
631/449/2679/2113
631/535/1266
Attraction
Gametophytes
Homology
Humanities and Social Sciences
Leucine
multidisciplinary
Mutagenesis
Peptides
Pollen
Pollen tubes
Receptors
Recognition
Reproduction (biology)
Rubella
Science
Science (multidisciplinary)
Sexual reproduction
Tubes
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Summary:Transportation of the immobile sperms directed by pollen tubes to the ovule-enclosed female gametophytes is important for plant sexual reproduction. The defensin-like (DEFL) cysteine-rich peptides (CRPs) LUREs play an essential role in pollen tube attraction to the ovule, though their receptors still remain controversial. Here we provide several lines of biochemical evidence showing that the extracellular domain of the leucine-rich repeat receptor kinase (LRR-RK) PRK6 from Arabidopsis thaliana directly interacts with AtLURE1 peptides. Structural study reveals that a C-terminal loop of the LRR domain (AtPRK6 LRR ) is responsible for recognition of AtLURE1.2, mediated by a set of residues largely conserved among PRK6 homologs from Arabidopsis lyrata and Capsella rubella , supported by in vitro mutagenesis and semi-in-vivo pollen tube growth assays. Our study provides evidence showing that PRK6 functions as a receptor of the LURE peptides in A. thaliana and reveals a unique ligand recognition mechanism of LRR-RKs. The cysteine-rich peptides LUREs play an essential role in pollen tube attraction to the ovule for plant sexual reproduction. Here Zhang et al. show that PRK6 functions as a receptor of the LUREs in Arabidopsis thaliana and reveal the ligand recognition mechanism.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-017-01323-8