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A New Strategy for Nucleic Acid Delivery and Protein Expression Using Biocompatible Nanohydrogels of Predefined Sizes

We have developed new formulations of nanohydrogels (NHGs) complexed with DNA devoid of cell toxicity, which, together with their tuned sizes, makes them of great interest for delivering DNA/RNA for foreign protein expression. Transfection results demonstrate that, unlike classical lipo/polyplexes,...

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Published in:Pharmaceutics 2023-03, Vol.15 (3), p.961
Main Authors: Eswaran, Lakshmanan, Kazimirsky, Gila, Yehuda, Ronen, Byk, Gerardo
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cited_by cdi_FETCH-LOGICAL-c534t-6aa70c39b1a1f59278bf3a754e8adbebadd3bc28acccf13ce05430f14b372a163
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description We have developed new formulations of nanohydrogels (NHGs) complexed with DNA devoid of cell toxicity, which, together with their tuned sizes, makes them of great interest for delivering DNA/RNA for foreign protein expression. Transfection results demonstrate that, unlike classical lipo/polyplexes, the new NHGs can be incubated indefinitely with cells without apparent cellular toxicity, resulting in the high expression of foreign proteins for long periods of time. Although protein expression starts with a delay as compared to classical systems, it is sustained for a long period of time, even after passing cells without observation of toxicity. A fluorescently labelled NHG used for gene delivery was detected inside cells very early after incubation, but the protein expression was delayed by many days, demonstrating that there is a time-dependent release of genes from the NHGs. We suggest that this delay is due to the slow but continuous release of DNA from the particles concomitantly with slow but continuous protein expression. Additionally, results obtained after the in vivo administration of m-Cherry/NHG complexes indicated a delayed but prolonged expression of the marker gene in the tissue of administration. Overall, we have demonstrated gene delivery and foreign protein expression using GFP and m-Cherry marker genes complexed with biocompatible nanohydrogels.
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identifier ISSN: 1999-4923
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source Publicly Available Content Database; PubMed Central; Coronavirus Research Database
subjects Acids
Alzheimer's disease
Biocompatibility
cationic nanohydrogels
Cell culture
Cell growth
Cloning
Dosage and administration
Gels (Pharmacy)
Gene expression
Gene therapy
Lipids
Materials
Microscopy
Nanomedicine
Nanoparticles
non-viral gene delivery
Plasmids
polymerization
Polymers in medicine
Proteins
self-assembly
Software
Structure
title A New Strategy for Nucleic Acid Delivery and Protein Expression Using Biocompatible Nanohydrogels of Predefined Sizes
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