Hypovirulence of Colletotrichum gloesporioides Associated with dsRNA Mycovirus Isolated from a Mango Orchard in Thailand

The pathogenic fungus Colletotrichum gloeosporioides causes anthracnose disease, which is an important fungal disease affecting the production of numerous crops around the world. The presence of mycoviruses, however, may have an impact on the pathogenicity of the fungal host. Here, we describe a dou...

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Bibliographic Details
Published in:Viruses 2022-08, Vol.14 (9), p.1921
Main Authors: Suharto, Aditya R., Jirakkakul, Jiraporn, Eusebio-Cope, Ana, Salaipeth, Lakha
Format: Article
Language:English
Subjects:
Anthracnose
anthracnose disease
Biological control
Cell division
Citrus fruits
Colletotrichum gloeosporioides
Disease
DNA-directed RNA polymerase
Double-stranded RNA
Enzymes
Ethanol
Fungi
Genomes
hypovirulence
Infections
Maximum likelihood method
Mycoviridae
mycovirus
Nucleotide sequence
Open reading frames
Pathogenicity
Phenols
Phylogenetics
Phylogeny
RNA viruses
RNA-directed RNA polymerase
Virulence
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Summary:The pathogenic fungus Colletotrichum gloeosporioides causes anthracnose disease, which is an important fungal disease affecting the production of numerous crops around the world. The presence of mycoviruses, however, may have an impact on the pathogenicity of the fungal host. Here, we describe a double-stranded RNA (dsRNA) mycovirus, which was isolated from a field strain of C. gloeosporioides, Ssa-44.1. The 2939 bp genome sequence comprises two open reading frames (ORFs) that encode for a putative protein and RNA-dependent RNA polymerase (RdRp). The Ssa-44.1 mycovirus is a member of the unclassified mycovirus family named Colletotrichum gloeosporioides RNA virus 1 strain Ssa-44.1 (CgRV1-Ssa-44.1), which has a phylogenetic similarity to Colletotrichum gleosporioides RNA virus 1 (CgRV1), which was isolated from citrus leaves in China. In C. gloeosporioides, CgRV1-Ssa-44.1 was shown to be linked to hypovirulence. CgRV1-Ssa-44.1 has a low spore transfer efficiency but can successfully spread horizontally to isogenic virus-free isolates. Furthermore, CgRV1-Ssa-44.1 had a strong biological control impact on C. gloeosporioides on mango plants. This study is the first to describe a hypovirulence-associated mycovirus infecting C. gloeosporioides, which has the potential to assist with anthracnose disease biological management.
ISSN:1999-4915
1999-4915
DOI:10.3390/v14091921