Genome-wide characterization and expression analysis of the Dof gene family related to abiotic stress in watermelon

The plant DNA-binding with one finger (Dof) gene family is a class of plant-specific transcription factors that play vital roles in many biological processes and stress responses. In the present study, a total of 36 genes were identified in the watermelon genome, which were unevenly distributed on 1...

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Published in:PeerJ (San Francisco, CA) CA), 2020-02, Vol.8, p.e8358-e8358, Article e8358
Main Authors: Zhou, Yong, Cheng, Yuan, Wan, Chunpeng, Li, Jingwen, Yang, Youxin, Chen, Jinyin
Format: Article
Language:English
Subjects:
Abiotic stress
Agricultural Science
Biosynthesis
Characterization
Chromosomes
Citrullus lanatus
Deoxyribonucleic acid
DNA
DNA binding proteins
Dof
Expression profile
Flowers & plants
Gene expression
Genes
Genetic aspects
Genomes
Genomics
Hormones
Phylogenetic analysis
Phylogenetics
Phylogeny
Plant Science
Polymerase chain reaction
Proteins
Seeds
Synteny
Transcription (Genetics)
Transcription factors
Watermelon
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Summary:The plant DNA-binding with one finger (Dof) gene family is a class of plant-specific transcription factors that play vital roles in many biological processes and stress responses. In the present study, a total of 36 genes were identified in the watermelon genome, which were unevenly distributed on 10 chromosomes. Phylogenetic analysis showed that the ClDof proteins could be divided into nine groups, and the members in a particular group had similar motif arrangement and exon-intron structure. Synteny analysis indicated the presence of a large number of syntenic relationship events between watermelon and cucumber. In promoter analysis, five kinds of stress-related and nine kinds of hormone-related -elements were identified in the promoter regions of genes. We then analyzed the expression patterns of nine selected genes in eight specific tissues by qRT-PCR, and the results showed that they have tissue-specific expression patterns. We also evaluated the expression levels of 12 selected genes under salt stress and ABA treatments using qRT-PCR. As a result, they showed differential expression under these treatments, suggesting their important roles in stress response. Taken together, our results provide a basis for future research on the biological functions of genes in watermelon.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.8358