Clonal diversity and detection of carbapenem resistance encoding genes among multidrug-resistant Acinetobacter baumannii isolates recovered from patients and environment in two intensive care units in a Moroccan hospital

Carbapenem-resistant has recently been defined by the World Health Organization as a critical pathogen. The aim of this study was to compare clonal diversity and carbapenemase-encoding genes of isolates collected from colonized or infected patients and hospital environment in two intensive care unit...

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Published in:Antimicrobial resistance & infection control 2017-09, Vol.6 (1), p.99-99, Article 99
Main Authors: Uwingabiye, Jean, Lemnouer, Abdelhay, Roca, Ignasi, Alouane, Tarek, Frikh, Mohammed, Belefquih, Bouchra, Bssaibis, Fatna, Maleb, Adil, Benlahlou, Yassine, Kassouati, Jalal, Doghmi, Nawfal, Bait, Abdelouahed, Haimeur, Charki, Louzi, Lhoussain, Ibrahimi, Azeddine, Vila, Jordi, Elouennass, Mostafa
Format: Article
Language:English
Subjects:
Acinetobacter
Acinetobacter Baumannii
Antibiotics
Bacteriology
blaNDM-1 gene
Deoxyribonucleic acid
Disease control
DNA
Drug resistance
Drug resistance in microorganisms
Environmental monitoring
Epidemics
Epidemiology
Gene expression
Genes
Genetic aspects
Genotype
Health aspects
Hospitals
Infections
Intensive care
Intensive care unit
Ionization
Laboratories
Mass spectrometry
Multidrug resistant organisms
Multidrug-resistant
OXA genes
Patients
Pulsed-field gel electrophoresis
Studies
Teaching hospitals
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Summary:Carbapenem-resistant has recently been defined by the World Health Organization as a critical pathogen. The aim of this study was to compare clonal diversity and carbapenemase-encoding genes of isolates collected from colonized or infected patients and hospital environment in two intensive care units (ICUs) in Morocco. The patient and environmental sampling was carried out in the medical and surgical ICUs of Mohammed V Military teaching hospital from March to August 2015. All isolates recovered from clinical and environmental samples, were identified using routine microbiological techniques and Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Antimicrobial susceptibility testing was performed using disc diffusion method. The carbapenemase-encoding genes were screened for by PCR. Clonal relatedness was analyzed by digestion of the DNA with low frequency restriction enzymes and pulsed field gel electrophoresis (PFGE) and the multi locus sequence typing (MLST) was performed on two selected isolates from two major pulsotypes. A total of 83 multidrug-resistant isolates were collected: 47 clinical isolates and 36 environmental isolates. All isolates were positive for the and genes. The coexistence of and were detected in 27 (32.5%) and 2 (2.4%) of isolates, respectively. The environmental samples and the fecally-colonized patients were significantly identified (  
ISSN:2047-2994
2047-2994
DOI:10.1186/s13756-017-0262-4