Adaptive Wave-Front Shaping and Beam Focusing through Fiber Bundles for High-Resolution Bioimaging

We demonstrate an adaptive wave-front shaping of optical beams transmitted through fiber bundles as a powerful resource for multisite, high-resolution bioimaging. With the phases of all the beamlets delivered through up to 6000 different fibers within the fiber bundle controlled individually, by mea...

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Bibliographic Details
Published in:Photonics 2022-01, Vol.9 (1), p.21
Main Authors: Pochechuev, Matvey S., Fedotov, Ilya V., Solotenkov, Maxim A., Andreeva, Maria S., Lanin, Aleksandr A., Fedotov, Andrei B., Zheltikov, Aleksei M.
Format: Article
Language:English
Subjects:
adaptive optics
Adaptive search techniques
bioimaging
Calcium imaging
Continuous fibers
Continuous wave lasers
Experiments
fiber-optics
Focusing
High definition
High resolution
Laser beams
Lasers
Medical imaging
Optics
Spatial discrimination
Spatial light modulators
Spatial resolution
Wave fronts
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Summary:We demonstrate an adaptive wave-front shaping of optical beams transmitted through fiber bundles as a powerful resource for multisite, high-resolution bioimaging. With the phases of all the beamlets delivered through up to 6000 different fibers within the fiber bundle controlled individually, by means of a high-definition spatial light modulator, the overall beam transmitted through the fiber bundle can be focused into a beam waist with a diameter less than 1 μm within a targeted area in a biotissue, providing a diffraction-limited spatial resolution adequate for single-cell or even subcellular bioimaging. The field intensity in the adaptively-focused continuous-wave laser beam in our fiber-bundle-imaging setting is more than two orders of magnitude higher than the intensity of the speckle background. Once robust beam focusing was achieved with a suitable phase profile across the input face of the fiber bundle, the beam focus can be scanned over a targeted area with no need for a further adaptive search, by applying a physically intuitive, wave-front-tilting phase mask on the field of input beamlets. This method of beam-focus scanning promises imaging speeds compatible with the requirements of in vivo calcium imaging.
ISSN:2304-6732
2304-6732
DOI:10.3390/photonics9010021