Optimization of Lipid Extraction from Spirulina spp. by Ultrasound Application and Mechanical Stirring Using the Taguchi Method of Experimental Design

The present study uses the Taguchi method of experimental design to optimize lipid extraction from Spirulina spp. by ultrasound application and mechanical stirring. A Taguchi L9 orthogonal array was used to optimize various parameters, such as methanol: chloroform (M:C) ratio, biomass: solvent ratio...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-10, Vol.27 (20), p.6794
Main Authors: Neag, Emilia, Stupar, Zamfira, Varaticeanu, Cerasel, Senila, Marin, Roman, Cecilia
Format: Article
Language:English
Subjects:
Algae
ANOVA
Biomass
Chloroform
Confidence intervals
Design of experiments
Design optimization
Ethanol
Experimental design
Fatty Acids
gamma-Linolenic Acid
Hypotheses
Linoleic Acid
Linolenic acid
Lipids
Methanol
orthogonal array
Orthogonal arrays
Palmitic acid
Palmitic Acids
Process parameters
Research Design
Signal to noise ratio
Solvent extraction processes
Solvents
Spirulina
Taguchi method
Taguchi methods
Ultrasonic imaging
Ultrasound
Variance analysis
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Summary:The present study uses the Taguchi method of experimental design to optimize lipid extraction from Spirulina spp. by ultrasound application and mechanical stirring. A Taguchi L9 orthogonal array was used to optimize various parameters, such as methanol: chloroform (M:C) ratio, biomass: solvent ratio, and extraction time for lipid extraction. The results were analyzed using the signal-to-noise (S/N) ratio and analysis of variance (ANOVA). The biomass: solvent ratio significantly influenced lipid content (p < 0.05) with 92.1% and 92.3% contributions to the lipid and S/N ratio data, respectively. The extraction time presented a contribution value of 5.0%, while the M:C ratio presented the most negligible contribution of 0.4% for S/N data. The optimum extraction conditions were: M:C ratio of 1:1, biomass: solvent ratio of 1:60, and extraction time of 30 min. The predominant fatty acids were palmitic acid (44.5%), linoleic acid (14.9%), and gamma-linolenic acid (13.4%). The confirmation experiments indicated a lipid content of 8.7%, within a 95% confidence interval, proving the Taguchi method’s effectiveness in optimizing the process parameters for lipid extraction.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27206794