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In planta proximity dependent biotin identification (BioID)
Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate...
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Published in: | Scientific reports 2018-06, Vol.8 (1), p.9212-8, Article 9212 |
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description | Proximity dependent biotin identification (BioID) has emerged as a powerful tool for studies of proteome architecture, including insoluble or membrane-associated proteins. The technique has been well established in mammalian cells but has yet to be applied to whole plant systems. Here we demonstrate the application of BioID on leaf tissues of the model plant
Arabidopsis thaliana
, thereby expanding the versatility of this important technique and providing a powerful proteomics tool for plant biologists. |
doi_str_mv | 10.1038/s41598-018-27500-3 |
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Arabidopsis thaliana
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Arabidopsis thaliana
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subjects | 631/449/1659 631/449/2169/2673 82 82/58 Biotin Datasets Humanities and Social Sciences Localization Mammalian cells Mass spectrometry multidisciplinary Plant tissues Protein expression Proteins Proteomics R&D Research & development Science Science (multidisciplinary) Scientific imaging |
title | In planta proximity dependent biotin identification (BioID) |
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