Comparison of cytosine base editors and development of the BEable-GPS database for targeting pathogenic SNVs

A variety of base editors have been developed to achieve C-to-T editing in different genomic contexts. Here, we compare a panel of five base editors on their C-to-T editing efficiencies and product purity at commonly editable sites, including some human pathogenic C-to-T mutations. We further profil...

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Bibliographic Details
Published in:Genome Biology 2019-10, Vol.20 (1), p.218-218, Article 218
Main Authors: Wang, Ying, Gao, Runze, Wu, Jing, Xiong, Yi-Chun, Wei, Jia, Zhang, Sipin, Yang, Bei, Chen, Jia, Yang, Li
Format: Article
Language:English
Subjects:
APOBEC Deaminases
Base editing
Base editor
Cell Line, Tumor
CRISPR-Cas Systems
CRISPR/Cas
Cytidine deaminase
Gene Editing - methods
Genomics - methods
Humans
Pathogenic mutation
Point Mutation
Short Report
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Summary:A variety of base editors have been developed to achieve C-to-T editing in different genomic contexts. Here, we compare a panel of five base editors on their C-to-T editing efficiencies and product purity at commonly editable sites, including some human pathogenic C-to-T mutations. We further profile the accessibilities of 20 base editors to all possible pathogenic mutations in silico. Finally, we build the BEable-GPS (Base Editable prediction of Global Pathogenic SNVs) database for users to select proper base editors to model or correct disease-related mutations. The in vivo comparison and in silico profiling catalog the availability of base editors and their broad applications in biomedical studies.
ISSN:1474-760X
1474-7596
1474-760X
DOI:10.1186/s13059-019-1839-4