Identification of Oxidative-Stress-Reducing Plant Extracts from a Novel Extract Library-Comparative Analysis of Cell-Free and Cell-Based In Vitro Assays to Quantitate Antioxidant Activity

Numerous underexplored plant species are believed to possess considerable potential in combating oxidative stress and its associated health impacts, emphasizing the need for a comprehensive methodological screening approach to assess their antioxidant capacity. This study investigated 375 plant extr...

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Published in:Antioxidants 2024-02, Vol.13 (3), p.297
Main Authors: Heckmann, Mara, Stadlbauer, Verena, Drotarova, Ivana, Gramatte, Theresa, Feichtinger, Michaela, Arnaut, Verena, Atzmüller, Stefanie, Schwarzinger, Bettina, Röhrl, Clemens, Blank-Landeshammer, Bernhard, Weghuber, Julian
Format: Article
Language:English
Subjects:
Analysis
antioxidant
Antioxidants
Biodiversity
Biological products
Carotenoids
Chronic illnesses
Cocoa
Comparative analysis
Ethylenediaminetetraacetic acid
extract library
Flowers & plants
Fragaria ananassa
Medicine, Botanic
Medicine, Herbal
Nitric oxide
Oxidation
Oxidative stress
Phenolic compounds
Plant extracts
Polyphenols
Raw materials
Reactive oxygen species
ROS
screening
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Summary:Numerous underexplored plant species are believed to possess considerable potential in combating oxidative stress and its associated health impacts, emphasizing the need for a comprehensive methodological screening approach to assess their antioxidant capacity. This study investigated 375 plant extracts, utilizing both cell-free and cellular methods to evaluate their antioxidant properties. Target-based antioxidant capacity was evaluated by the total phenolic content (TPC) and ferric reducing antioxidant power (FRAP) assays. Cell-based assays employed the H DCF-DA probe to measure reactive oxygen species (ROS) levels and the Griess assay to quantify nitric oxide (NO) levels in stressed Caco-2 and RAW264.7 cells, respectively. The highest TPC and FRAP values were found in extracts of and leaves. Several plant extracts significantly reduced stress-induced ROS or NO levels by at least 30%. Distinctive selectivity was noted in certain extracts, favoring the significant reduction of NO (e.g., extract), of ROS (e.g., subsp. extract), or of both (e.g., extract). A strong correlation between TPC and FRAP values and moderate correlations between the results of the cell-free and cell-based assays were evident. These findings highlight the great antioxidant potential of underexplored plant extracts and the diversity of the underlying mechanisms, emphasizing the importance of a multifaceted approach for a comprehensive assessment.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox13030297