Biological Characteristics of the Mycelium and Optimization of the Culture Medium for Phallus dongsun

• Published in: Polish journal of microbiology 2024-06, Vol.73 (2), p.237-252
• Main Authors: Kang, Chao, Zheng, Xuan, Wang, Wankun, Zeng, Weijun, Wang, Jing, Liu, Zhongxuan, Yang, Ling, Wang, Fang, Zhu, Yan
• Format: Article
• Language: English
• Subjects: Ammonium chloride; Breeding; Carbon - chemistry; Carbon sources; Culture media; Culture Media - chemistry; Growth rate; Inorganic salts; Mycelia; Mycelium - growth & development; mycelium growth rate; Nitrogen; Nitrogen sources; nutrient composition; Original Paper; phallus dongsun; Polysaccharides, Bacterial - chemistry; Powder; Response surface methodology; Soybeans; suspension agents; Temperature; temperature gradient; Xanthan; Xanthan gum; Zinc sulfate
• ISSN: 2544-4646; 1733-1331; 2544-4646
• DOI: 10.33073/pjm-2024-022

This study aimed to elucidate the influence of various culture medium components, including carbon sources, nitrogen sources, inorganic salts, suspension agents, and temperature, on the mycelial growth characteristics of . Employing single-factor experiments and response surface methodology within glass Petri dishes, the research identified that carrot powder, soybean powder, and ZnSO notably enhanced the proliferation of aerial mycelium, significantly augmenting the growth rate of mycelium. The resultant mycelium was observed to be dense, robust, and fluffy in texture. In particular, ZnSO markedly accelerated the mycelium growth rate. Furthermore, xanthan gum was found to effectively modulate the medium's viscosity, ensuring a stable suspension and facilitating nutrient equilibrium. The optimal cultivation temperature was determined to be 25°C, with mycelial growth ceasing below 5°C and mycelium perishing at temperatures exceeding 35°C. The optimal medium composition was established as follows: wheat starch 5 g/l, carrot powder 5 g/l, soybean powder 7.50 g/l, glucose 10 g/l, ZnSO 0.71 g/l, NH Cl 0.68 g/l, xanthan gum 0.5 g/l, and agar 20 g/l. Under these optimized conditions, the mycelium of exhibited a rapid growth rate (1.04 ± 0.14 mm/day), characterized by a thick, dense, and well-developed structure. This investigation provides a theoretical foundation for the conservation, strain selection, and breeding of .