Good agreements between self and clinician-collected specimens for the detection of human papillomavirus in Brazilian patients

• Published in: Memórias do Instituto Oswaldo Cruz 2014-06, Vol.109 (3), p.352-355
• Main Authors: de Campos, Karla Lopes Mandu, Machado, Ana Paula, de Almeida, Flávia Gatto, Bonin, Camila Mareti, Prata, Thiago Theodoro Martins, Almeida, Larissa Zatorre, Padovani, Cacilda Tezelli Junqueira, Ferreira, Alda Maria Teixeira, Fernandes, Carlos Eurico dos Santos, Tozetti, Inês Aparecida
• Format: Article
• Language: English
• Subjects: Adult; Aged; Cervix Uteri - virology; clinician-collected; DNA, Viral - analysis; Female; Genotype; human papillomavirus; Humans; Middle Aged; Papillomaviridae - genetics; Papillomaviridae - isolation & purification; Papillomavirus Infections - diagnosis; PARASITOLOGY; PCR; Polymerase Chain Reaction; Self Care - methods; self-collected; Sensitivity and Specificity; Specimen Handling - methods; TROPICAL MEDICINE; Young Adult
• ISSN: 1678-8060; 0074-0276; 1678-8060
• DOI: 10.1590/0074-0276130397

Women infected with human papillomavirus (HPV) are at a higher risk of developing cervical lesions. In the current study, self and clinician-collected vaginal and cervical samples from women were processed to detect HPV DNA using polymerase chain reaction (PCR) with PGMY09/11 primers. HPV genotypes were determined using type-specific PCR. HPV DNA detection showed good concordance between self and clinician-collected samples (84.6%; kappa = 0.72). HPV infection was found in 30% women and genotyping was more concordant among high-risk HPV (HR-HPV) than low-risk HPV (HR-HPV). HPV16 was the most frequently detected among the HR-HPV types. LR-HPV was detected at a higher frequency in self-collected; however, HR-HPV types were more frequently identified in clinician-collected samples than in self-collected samples. HPV infections of multiple types were detected in 20.5% of clinician-collected samples and 15.5% of self-collected samples. In this study, we demonstrated that the HPV DNA detection rate in self-collected samples has good agreement with that of clinician-collected samples. Self-collected sampling, as a primary prevention strategy in countries with few resources, could be effective for identifying cases of HR-HPV, being more acceptable. The use of this method would enhance the coverage of screening programs for cervical cancer.