Effect of Trilobatin from Lithocarpus polystachyus Rehd on Gut Microbiota of Obese Rats Induced by a High-Fat Diet

• Published in: Nutrients 2021-03, Vol.13 (3), p.891
• Main Authors: Shen, Hailiang, Huang, Linhua, Dou, Huating, Yang, Yali, Wu, Houjiu
• Format: Article
• Language: English
• Subjects: Abundance; Animals; Anti-Obesity Agents - administration & dosage; Anti-Obesity Agents - pharmacology; antiobesity; Bacteroidetes - classification; Bacteroidetes - growth & development; Body Weight - drug effects; Body weight gain; Cardiovascular disease; Chromatography; Diet; Diet, High-Fat; Dietary Supplements; Discriminant analysis; Drug dosages; Drug therapy; Encyclopedias; Fagaceae - chemistry; Fatty Acids, Volatile - analysis; Firmicutes - classification; Firmicutes - growth & development; Flavonoids; Flavonoids - administration & dosage; Flavonoids - pharmacology; Food; Gastrointestinal Microbiome - drug effects; Genomes; gut microbiota; High fat diet; Intestinal microflora; KEGG pathway; Lactobacillus; Lithocarpus; Lithocarpus polystachyus Rehd; Liver - drug effects; Male; Metabolic Networks and Pathways - drug effects; Metabolic pathways; Metabolism; Microbiota; Obesity; Obesity - etiology; Obesity - metabolism; Obesity - microbiology; Organ Size - drug effects; Phylogeny; Plant Leaves - chemistry; Polyphenols - administration & dosage; Polyphenols - pharmacology; Principal components analysis; Rats; Rats, Sprague-Dawley; Relative abundance; Rodents; trilobatin
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu13030891

Trilobatin was identified as the primary bioactive component in the Rehd (LPR) leaves. This study explored the antiobesity effect of trilobatin from LPR leaves and its influence on gut microbiota in obese rats. Results showed that trilobatin could significantly reduce body and liver weight gain induced by a high-fat diet, and the accumulation of perirenal fat, epididymal fat, and brown fat of SD (Male Sprague-Dawley) obese rats in a dose-independent manner. Short-chain fatty acids (SCFAs) concentrations increased, especially the concentration of butyrate. Trilobatin supplementation could significantly increase the relative abundance of , , , , and , and decrease greatly the abundance of , , and , resulting in an increase of the ratio of to (except the genera of and ). The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway predicted by the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) indicated the different relative metabolic pathways after trilobatin supplementation. This study may reveal the contribution of gut microbiota to the antiobesity effect of trilobatin from LPR leaves and predict the potential regulatory mechanism for obesity induced by a high-fat diet.