In vitro evaluation of Dracocephalum lindbergii on growth and apoptosis induction against Leishmania major and cytokine analysis

This study aims to assess the in vitro anti-Leishmania activity of Dracocephalum lindbergii (D. lindbergii) and its essential oils. The plant, belonging to the Lamiaceae family, has a history of traditional medicinal use. Leishmaniasis, a vector-borne disease caused by protozoan parasites of the gen...

Full description

Saved in:
Bibliographic Details
Published in:Journal of global antimicrobial resistance. 2024-12, Vol.39, p.43-43
Main Authors: Khamesipour, Faham, Khamesipour, Ali, Hejazi, Seyed Hossein, Ghanadian, Mustafa
Format: Article
Language:English
Subjects:
Apoptosis
Dracocephalum lindbergii
Leishmania major and promastigotes
Traditionnel medicine
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:This study aims to assess the in vitro anti-Leishmania activity of Dracocephalum lindbergii (D. lindbergii) and its essential oils. The plant, belonging to the Lamiaceae family, has a history of traditional medicinal use. Leishmaniasis, a vector-borne disease caused by protozoan parasites of the genus Leishmania, poses significant health risks. Aerial parts of D. lindbergii were collected from Iran, and essential components were extracted using a series of solvents. Gas chromatography coupled with mass spectrometry was utilized for component analysis. The impact of D. lindbergii fractions and essential oil on Leishmania major promastigotes, amastigotes, and macrophages was assessed using the MTT method for cell viability and flow cytometry for apoptosis. Real-time PCR quantified cytokine expression on murine macrophages. Results show the essential oil's potent anti-Leishmania activity against amastigotes after 24 hours. Fraction F1 was most effective against promastigotes, while F3 showed high activity against macrophages with a favorable selectivity index. Time-dependent variations in activity were observed. Cellular morphology changes included shrinkage and cytoplasmic condensation after treatment. Fraction F4 exhibited notable antioxidant properties. The results also indicated time-dependent variations in the anti-Leishmania activity of the essential oil and the fractions. D. lindbergii and its essential oils hold promise as anti-Leishmania agents, with the essential oil and F4 fraction being potential candidates for drug development. Further research and clinical trials are necessary to explore their therapeutic potential fully.
ISSN:2213-7165
DOI:10.1016/j.jgar.2024.10.138