New dual-function in situ bone repair scaffolds promote osteogenesis and reduce infection

The treatment of infectious bone defects is a difficult problem to be solved in the clinic. In situ bone defect repair scaffolds with anti-infection and osteogenic abilities can effectively deal with infectious bone defects. In this study, an in situ polycaprolactone (PCL) scaffold containing ampici...

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Bibliographic Details
Published in:Journal of biological engineering 2022-09, Vol.16 (1), p.1-23, Article 23
Main Authors: Yang, Changsheng, Zhou, Lei, Geng, Xiaodan, Zhang, Hui, Wang, Baolong, Ning, Bin
Format: Article
Language:English
Subjects:
3-D printers
3D printing
Ampicillin
Antibiotics
Antiinfectives and antibacterials
Bacteria
Bone healing
Cbfa-1 protein
Cell adhesion
Cell adhesion & migration
Cell culture
Collagen
Collagen (type I)
Composite materials
Contact angle
Defects
Dual-function
E coli
Gram-positive bacteria
Growth factors
Health aspects
In situ
Infection
Infections
Infectious bone defect
Magnesium
Methicillin
MicroRNAs
Microspheres
Morphology
Osteogenesis
Penicillin
Peptides
Polycaprolactone
Scaffolds
Skin & tissue grafts
Staphylococcus infections
Stem cells
Three dimensional printing
Tissue engineering
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Summary:The treatment of infectious bone defects is a difficult problem to be solved in the clinic. In situ bone defect repair scaffolds with anti-infection and osteogenic abilities can effectively deal with infectious bone defects. In this study, an in situ polycaprolactone (PCL) scaffold containing ampicillin (Amp) and Mg microspheres was prepared by 3D printing technology. Mg and Amp were evenly distributed in PCL scaffolds and could be released slowly to the surrounding defect sites with the degradation of scaffolds. In vitro experiments demonstrated that the PCL scaffold containing Mg and Amp (PCL@Mg/Amp) demonstrated good cell adhesion and proliferation. The osteogenic genes collagen I (COL-I) and Runx2 were upregulated in cells grown on the PCL@Mg/Amp scaffold. The PCL@Mg/Amp scaffold also demonstrated excellent antibacterial ability against E. coli and S. aureus. In vivo experiments showed that the PCL@Mg/Amp scaffold had the strongest ability to promote tibial defect repair in rats compared with the other groups of scaffolds. This kind of dual-function in situ bone repair scaffold with anti-infection and osteogenic abilities has good application prospects in the field of treating infectious bone defects.
ISSN:1754-1611
1754-1611
DOI:10.1186/s13036-022-00302-y