Divergence in the Regulation of the Salt Tolerant Response Between Arabidopsis thaliana and Its Halophytic Relative Eutrema salsugineum by mRNA Alternative Polyadenylation

• Published in: Frontiers in plant science 2022-03, Vol.13, p.866054-866054
• Main Authors: Ma, Hui, Cai, Lingling, Lin, Juncheng, Zhou, Kaiyue, Li, Qingshun Q
• Format: Article
• Language: English
• Subjects: alternative polyadenylation; Arabidopsis thaliana; Eutrema salsugineum; PAT-seq; Plant Science; RNA processing; salt tolerance
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2022.866054

Salt tolerance is an important mechanism by which plants can adapt to a saline environment. To understand the process of salt tolerance, we performed global analyses of mRNA alternative polyadenylation (APA), an important regulatory mechanism during eukaryotic gene expression, in and its halophytic relative with regard to their responses to salt stress. Analyses showed that while APA occurs commonly in both and , possesses fewer APA genes than (47% vs. 54%). However, the proportion of APA genes was significantly increased in under salt stress but not in . This indicated that is more sensitive to salt stress and that exhibits an innate response to such conditions. Both species utilized distal poly(A) sites under salt stress; however, only eight genes were found to overlap when their 3' untranslated region (UTR) lengthen genes were compared, thus revealing their distinct responses to salt stress. In , genes that use distal poly(A) sites were enriched in response to salt stress. However, in , the use of poly(A) sites was less affected and fewer genes were enriched. The transcripts with upregulated poly(A) sites in showed enriched pathways in plant hormone signal transduction, starch and sucrose metabolism, and fatty acid elongation; in , biosynthetic pathways (stilbenoid, diarylheptanoid, and gingerol) and metabolic pathways (arginine and proline) showed enrichment. APA was associated with 42% and 29% of the differentially expressed genes (DE genes) in and experiencing salt stress, respectively. Salt specific poly(A) sites and salt-inducible APA events were identified in both species; notably, some salt tolerance-related genes and transcription factor genes exhibited differential APA patterns, such as and . Our results suggest that adapted species exhibit more orderly response at the RNA maturation step under salt stress, while more salt-specific poly(A) sites were activated in to cope with salinity conditions. Collectively, our findings not only highlight the importance of APA in the regulation of gene expression in response to salt stress, but also provide a new perspective on how salt-sensitive and salt-tolerant species perform differently under stress conditions through transcriptome diversity.