Characterization of pathogenic microbiome on removable prostheses with different levels of cleanliness using 2bRAD-M metagenomic sequencing

The microbiomes on the surface of unclean removable prostheses are complex and yet largely underexplored using metagenomic sequencing technology. To characterize the microbiome of removable prostheses with different levels of cleanliness using Type IIB Restriction-site Associated DNA for Microbiome...

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Bibliographic Details
Published in:Journal of oral microbiology 2024, Vol.16 (1), p.2317059-2317059
Main Authors: Lim, Tong Wah, Huang, Shi, Jiang, Yuesong, Zhang, Yufeng, Burrow, Michael Francis, McGrath, Colman
Format: Article
Language:English
Subjects:
Bacteria
Cleanliness
DNA sequencing
Gene sequencing
Metagenomics
microbial index
Microbiomes
Microorganisms
pathogenic bacteria
Prostheses
prosthesis cleanliness
Prosthetics
Qualitative analysis
Relative abundance
Removable prosthesis
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Summary:The microbiomes on the surface of unclean removable prostheses are complex and yet largely underexplored using metagenomic sequencing technology. To characterize the microbiome of removable prostheses with different levels of cleanliness using Type IIB Restriction-site Associated DNA for Microbiome (2bRAD-M) sequencing and compare the Microbial Index of Pathogenic Bacteria (MIP) between clean and unclean prostheses. Ninety-seven removable prostheses were classified into 'clean' and 'unclean' groups. All prosthesis plaque samples underwent 2bRAD metagenomic sequencing to characterize the species-resolved microbial composition. MIPs for clean and unclean prostheses were calculated based on the sum of the relative abundance of pathogenic bacteria in a microbiome using a reference database that contains opportunistic pathogenic bacteria and disease-associated information. Beta diversity analyses based on Jaccard qualitative and Bray-Curtis quantitative distance matrices identified significant differences between the two groups (  
ISSN:2000-2297
2000-2297
DOI:10.1080/20002297.2024.2317059