Expression of Hsp60 and its cell location in Paracoccidioides brasiliensis

Paracoccidioides species cause paracoccidioidomycosis (PCM), a systemic mycosis highly prevalent in Brazil. Therapy of PCM has some issues that make studies for new therapeutic and vaccine targets relevant, such as the P. brasiliensis 60-kDa-heat-shock protein (PbHsp60), an immunogenic antigen that...

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Published in:Revista do Instituto de Medicina Tropical de São Paulo 2020-01, Vol.62, p.e29-6
Main Authors: Fernandes, Fabrício Freitas, Pitangui, Nayla S, Peron, Gabriela, Valim, Clarissa X R, Mazucato, Vívian M, Oliveira, Aline F, Silva, Thiago A da, Roque-Barreira, Maria-Cristina, Panunto-Castelo, Ademilson
Format: Article
Language:English
Subjects:
Animals
Antigens, Fungal - immunology
Brief Communication
Chaperonin 60 - immunology
Gene Expression - immunology
heat-shock proteins
hsp60
Male
Mice
Mice, Inbred BALB C
Paracoccidioides - immunology
Paracoccidioides - pathogenicity
paracoccidioides brasiliensis
Polymerase Chain Reaction
RNA, Messenger - immunology
subcellular localization
TROPICAL MEDICINE
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Summary:Paracoccidioides species cause paracoccidioidomycosis (PCM), a systemic mycosis highly prevalent in Brazil. Therapy of PCM has some issues that make studies for new therapeutic and vaccine targets relevant, such as the P. brasiliensis 60-kDa-heat-shock protein (PbHsp60), an immunogenic antigen that induces protection in experimental mice infection. Here, we investigated the relative expression of mRNA for PbHsp60 in P. brasiliensis in the different morphotypes of P. brasiliensis and in morphological transition phases. In addition, antibodies to rPbHsp60 were produced and used to analyze the location of PbHsp60 in yeast and hyphae by electron microscopy. The analyses showed a substantial increase in the relative amounts of HSP60 mRNA in yeast when compared to mycelium and an intermediate expression in transitional forms. Regarding the cell location, immunoelectron microscopy analysis revealed that PbHsp60 is within the cell wall. These observations suggest that this protein may be involved in the maintenance of the cell wall integrity and the interaction with the host for colonization, infection and pathogenesis.
ISSN:1678-9946
0036-4665
1678-9946
DOI:10.1590/S1678-9946202062029