Quantity of glucosinolates in 10 cabbage genotypes and their impact on the feeding of Mamestra brassicae caterpillars

• Published in: Archives of biological sciences 2014, Vol.66 (2), p.867-876
• Main Authors: Bohinc, Tanja, Devetak, M., Trdan, S.
• Format: Article
• Language: English
• Subjects: cabbage; feeding preference; genotype; glucosinolates; Mamestra brassicae
• ISSN: 0354-4664; 1821-4339
• DOI: 10.2298/ABS1402867B

In 2011, we studied the glucosinolate content in 5 cultivars and 5 cabbage hybrids grown outdoors in order to study their influence on the feeding of cabbage moth caterpillars (Mamestra brassicae). The selected genotypes were categorized into three groups, early (the growth period from 55 to 70 days), mid-early (80-90 days) and mid-late (110-140 days), while the samples of cabbage for glucosinolate analysis were taken at five intervals, during which we also assessed genotypes for the extent of damage caused by caterpillars. We found that the feeding of caterpillars affected primarily the mid-early and mid-late genotypes of cabbage, and that the glucosinolate content among the different cabbage genotypes varies. The highest content of the analyzed glucosinolates was established in mid-late genotypes. Glucobrassicin was the only glucosinolate found in all cabbage genotypes, yet its antixenotic effect (r=0.20) was very low. We found that sinalbin negatively affects the feeding of cabbage moth caterpillars in mid-early cabbage genotypes (r=-0.34), while the same effect of sinigrin on the extent of damage can be observed in mid-late genotypes (r=-0.27). We have established a strong or moderate correlation between the gluconapin (r=0.87) and progoitrin (r=0.66) contents in mid-late genotypes and the extent of damage caused by caterpillars. Our research proves that different cabbage genotypes are responsible for different susceptibilities to damage by the cabbage moth, and that one of the factors of natural resistance of cabbage are also glucosinolates. Despite this, due to their variability in cabbage we cannot precisely determine the set of genotypes that would ensure a higher cabbage yield as a result of less damage caused by the cabbage moth. Thus, we need to identify in more detail the reasons for the time and quantum variability of glucosinolates in Brassicaceae. nema